Anti-Phospho-GABAA Receptor gamma2 (Ser327) GABRG2 Antibody

Product Name

Anti-Phospho-GABAA Receptor gamma2 (Ser327) GABRG2 Antibody

View all GABA-A R gamma 2 Antibodies

SKU/Catalog Number

P02361

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-GABAA Receptor gamma2 (Ser327) GABRG2 Antibody (Catalog # P02361). Tested in WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-Phospho-GABAA Receptor gamma2 (Ser327) GABRG2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P02361)

Host

Rabbit

Contents

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser327 of the γ2 subunit of rat GABAA receptor, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P02361 is reactive to GABRG2 in Mouse, Rat

Applications

P02361 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

54162 MW

Background of GABA-A R gamma 2

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABA-A and the GABA-AB subtype of receptors. GABA-A-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABA-A-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABA-A-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. It has recently been suggested that PKCε regulates the sensitivity of GABA-A α1β2γ2 receptors to ethanol and benzodiazepines through phosphorylation of serine 327 in the large intracellular loop of γ2 (Qi et al., 2007)

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000

Validation Images & Assay Conditions

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Western blot of rat cortical lysate showing specific immunolabeling of the ~45 kDa GABA_Agamma₂ protein phosphorylated at Ser³²⁷ in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Gene/Protein Information For GABRG2 (Source: Uniprot.org, NCBI)

Alternative Names

CAE2; ECA2; GABA A R gamma 2; GABA(A) receptor subunit gamma-2; GABAAR gamma 2; GABAARg2; GABRG2; gamma-aminobutyric acid (GABA) A receptor, gamma 2; gamma-aminobutyric acid receptor subunit gamma-2; GEFSP3 GABRG2 CAE2, DEE74, ECA2, EIEE74, FEB8, GEFSP3 gamma-aminobutyric acid type A receptor subunit gamma2 gamma-aminobutyric acid receptor subunit gamma-2|GABA(A) receptor subunit gamma-2|GABA(A) receptor, gamma 2|gamma-aminobutyric acid (GABA) A receptor, gamma 2|gamma-aminobutyric acid type A receptor gamma2 subunit

For more info on GABRG2, check out the GABRG2 Infographic

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In this infographic, you will see the following information for GABRG2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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