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Product Info Summary
| SKU: | A01989-2 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Gab1 (E655) Antibody
SKU/Catalog Number
A01989-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Gab1 (E655) Antibody catalog # A01989-2. Tested in WB,IHC,IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Gab1 (E655) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01989-2)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Immunogen
Synthetic peptide, corresponding to amino acids 622-672 of Human Gab1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01989-2 is reactive to GAB1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
76616 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01989-2 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
IF: 1:50-1:200
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of GAB1 using Anti-Gab1 (E655) Antibody (A01989-2).
Immunohistochemistry (IHC) analyzes of Gab1 (E655) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.For more protocol information of IHC
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Figure 1. Western blotting validation for Anti-Gab1 (E655) Antibody A01989-2
Western blot (WB) analysis of Gab1 (E655) polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysate
Lane2:NIH-3T3 whole cell lysate
Lane3:H9C2 whole cell lysate
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
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Figure 3. Western blotting validation for Anti-Gab1 (E655) Antibody A01989-2
Western blot (WB) analysis of Gab1 (E655) pAb at 1:500 dilutionLane1:A549 whole cell lysate(40ug)
Lane2:L02 whole cell lysate(40ug)
Lane3:The heart tissue lysate of Mouse(40ug)
Lane4:The heart tissue lysate of Rat(40ug)
Lane5:The brain tissue lysate of Mouse(40ug)
Lane6:The brain tissue lysate of Rat(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For GAB1 (Source: Uniprot.org, NCBI)
Gene Name
GAB1
Full Name
GRB2-associated-binding protein 1
Weight
76616 MW
Superfamily
GAB family
Alternative Names
GRB2-associated-binding protein 1;GRB2-associated binder 1;Growth factor receptor bound protein 2-associated protein 1;GAB1; GAB1 DFNB26 GRB2 associated binding protein 1 GRB2-associated-binding protein 1|GRB2-associated binder 1|deafness, autosomal recessive 26|growth factor receptor bound protein 2-associated protein 1|symbol withdrawn, see GAB1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GAB1, check out the GAB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GAB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Gab1 (E655) Antibody (A01989-2)
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