Click image to see more details
Product Info Summary
| SKU: | A01329-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-FOXP2 Antibody Picoband®
SKU/Catalog Number
A01329-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FOXP2 Antibody Picoband® catalog # A01329-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FOXP2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01329-1)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human FOXP2 recombinant protein (Position: L637-E715). Human FOXP2 shares 100% amino acid (aa) sequence identity with both mouse and rat FOXP2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01329-1 is reactive to FOXP2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
90 kDa
Calculated molecular weight
79919 MW
Background of FOXP2
Forkhead box protein P2 (FOXP2) is a protein that, in humans, is encoded by the FOXP2 gene. This gene encodes a member of the forkhead/winged-helix (FOX) family of transcription factors. It is expressed in fetal and adult brain as well as in several other organs such as the lung and gut. The protein product contains a FOX DNA-binding domain and a large polyglutamine tract and is an evolutionarily conserved transcription factor, which may bind directly to approximately 300 to 400 gene promoters in the human genome to regulate the expression of a variety of genes. This gene is required for proper development of speech and language regions of the brain during embryogenesis, and may be involved in a variety of biological pathways and cascades that may ultimately influence language development. Mutations in this gene cause speech-language disorder 1 (SPCH1), also known as autosomal dominant speech and language disorder with orofacial dyspraxia.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01329-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: rat brain tissue, mouse brain tissue, human SK-OV-3 whole Cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of FOXP2 using anti-FOXP2 antibody (A01329-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysate,
Lane 2: mouse brain tissue lysate,
Lane 3: human SK-OV-3 whole Cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXP2 antigen affinity purified polyclonal antibody (Catalog # A01329-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXP2 at approximately 90KD. The expected band size for FOXP2 is at 80KD.
Protein Target Info & Infographic
Gene/Protein Information For FOXP2 (Source: Uniprot.org, NCBI)
Gene Name
FOXP2
Full Name
Forkhead box protein P2
Weight
79919 MW
Alternative Names
Forkhead box protein P2;CAG repeat protein 44;Trinucleotide repeat-containing gene 10 protein;FOXP2;CAGH44, TNRC10; FOXP2 CAGH44, SPCH1, TNRC10 forkhead box P2 forkhead box protein P2|CAG repeat protein 44|forkhead/winged-helix transcription factor|trinucleotide repeat containing 10|trinucleotide repeat-containing gene 10 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FOXP2, check out the FOXP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FOXP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FOXP2 Antibody Picoband® (A01329-1)
Hello CJ!
No publications found for A01329-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-FOXP2 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-FOXP2 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Anti-FOXP2 Antibody Picoband®
Question
I was wanting to use your anti-FOXP2 antibody for WB for rat brain cortex on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat brain cortex identification?
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
It shows on the product datasheet, A01329-1 anti-FOXP2 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain cortex in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-16
Question
Is this A01329-1 anti-FOXP2 antibody reactive to the isotypes of FOXP2?
Verified Customer
Verified customer
Asked: 2019-01-17
Answer
The immunogen of A01329-1 anti-FOXP2 antibody is E. coli-derived human FOXP2 recombinant protein (Position: L637-E715). Human FOXP2 shares 100% amino acid (aa) sequence identity with both mouse and rat FOXP2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-01-17
Question
We are currently using anti-FOXP2 antibody A01329-1 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2018-06-29
Answer
The anti-FOXP2 antibody (A01329-1) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-06-29
Question
I am interested in to test anti-FOXP2 antibody A01329-1 on rat brain cortex for research purposes, then I may be interested in using anti-FOXP2 antibody A01329-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-06-04
Answer
The products we sell, including anti-FOXP2 antibody A01329-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-06-04
Question
I have attached the WB image, lot number and protocol we used for brain cortex using anti-FOXP2 antibody A01329-1. Please let me know if you require anything else.
A. Krishna
Verified customer
Asked: 2013-10-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-10-17
