Anti-TRAM2 Antibody Picoband®

Product Name

Anti-TRAM2 Antibody Picoband®

View all TRAM2 Antibodies

SKU/Catalog Number

PA2011

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TRAM2 Antibody catalog # PA2011. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TRAM2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2011)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of mouse TRAM2, identical to the related rat sequence, and different from the related human sequence by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2011 is reactive to Tram2 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

56 kDa

Calculated molecular weight

43183 MW

Background of TRAM2

TRAM2 (Translocation-Associating Membrane Protein 2), also called KIAA0057, is a protein that in humans is encoded by the TRAM2 gene. TRAM2 is a component of the translocon, a gated macromolecular channel that controls the posttranslational processing of nascent secretory and membrane proteins at the endoplasmic reticulum (ER) membrane. By genomic sequence analysis, Onuchic et al. (1999) mapped the TRAM2 gene to chromosome 6p21.1-p12 near the PKHD1 gene. Upon activation, quiescent hepatic stellate cells proliferate, change morphologically into myofibroblasts, and increase their synthesis of extracellular matrix proteins. Stefanovic et al. (2004) demonstrated that both TRAM2 and collagen type I are upregulated in activated rat and human hepatic stellate cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA2011 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse, Human, Rat

Validation Images & Assay Conditions

Gene/Protein Information For Tram2 (Source: Uniprot.org, NCBI)

Alternative Names

Translocating chain-associated membrane protein 2;Tram2; TRAM2 translocation associated membrane protein 2 translocating chain-associated membrane protein 2|TRAM-like protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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