Anti-Forkhead box protein P1 FOXP1 Antibody Picoband®

Product Name

Anti-Forkhead box protein P1 FOXP1 Antibody Picoband®

View all FOXP1 Antibodies

SKU/Catalog Number

PA1593

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Forkhead box protein P1 FOXP1 Antibody catalog # PA1593. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Forkhead box protein P1 FOXP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1593)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human FOXP1,identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1593 is reactive to FOXP1 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

84 kDa

Calculated molecular weight

75317 MW

Background of FOXP1

FOXP1 ("forkhead box P1") is a gene that is necessary for the proper development of the brain and lung in mammals. It is a member of the large FOX family of transcription factors. This gene belongs to subfamily P of the forkhead box (FOX) transcription factor family. Forkhead box transcription factors play important roles in the regulation of tissue- and cell type-specific gene transcription during both development and adulthood. Forkhead box P1 protein contains both DNA-binding- and protein-protein binding-domains. This gene may act as a tumor suppressor as it is lost in several tumor types and maps to a chromosomal region (3p14.1) reported to contain a tumor suppressor gene (s). Alternative splicing results in multiple transcript variants encoding different isoforms. It was shown that the embryonic stem cell (ESC)-specific isoform of FOXP1 stimulates the expression of transcription factor genes required for pluripotency, including OCT4, NANOG, NR5A2, and GDF3, while concomitantly repressing genes required for ESC differentiation. This isoform also promotes the maintenance of ESC pluripotency and contributes to efficient reprogramming of somatic cells into induced pluripotent stem cells. These results reveal a pivotal role for an Alternative splicing event in the regulation of pluripotency through the control of critical ESC-specific transcriptional programs (2).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

PA1593 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Positive Control

WB: Rat Spleen Tissue, COLO320 Cell
IHC: Human Rectal Cancer Tissue

Validation Images & Assay Conditions

Gene/Protein Information For FOXP1 (Source: Uniprot.org, NCBI)

Alternative Names

Forkhead box protein P1;Mac-1-regulated forkhead ;MFH ;FOXP1;HSPC215; FOXP1 12CC4, HSPC215, MFH, QRF1, hFKH1B forkhead box P1 forkhead box protein P1|fork head-related protein like B|glutamine-rich factor 1|mac-1-regulated forkhead

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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