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Product Info Summary
| SKU: | A00142 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Bcl-6 Antibody
SKU/Catalog Number
A00142
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Bcl-6 Antibody catalog # A00142. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Bcl-6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00142)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human BCL6. AA range:271-320
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00142 is reactive to BCL6 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
78846 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00142 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
IHC-P, 1:100-1:300
Validation Images & Assay Conditions
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Western Blot (WB) analysis of 293 cells using Bcl-6 Polyclonal antibody.
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Immunohistochemistry (IHC) analysis of paraffin-embedded Human Liver, antibody was diluted at 1:100.
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Immunohistochemistry (IHC) analysis of paraffin-embedded Human Liver, antibody was diluted at 1:100.
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Immunohistochemistry (IHC) analysis of paraffin-embedded Human Liver, antibody was diluted at 1:100.
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Immunohistochemistry (IHC) analysis of paraffin-embedded Human Liver, antibody was diluted at 1:100.
Protein Target Info & Infographic
Gene/Protein Information For BCL6 (Source: Uniprot.org, NCBI)
Gene Name
BCL6
Full Name
B-cell lymphoma 6 protein
Weight
78846 MW
Alternative Names
B-cell lymphoma 6 protein;BCL-6;B-cell lymphoma 5 protein;BCL-5;Protein LAZ-3;Zinc finger and BTB domain-containing protein 27;Zinc finger protein 51;BCL6;BCL5, LAZ3, ZBTB27, ZNF51; BCL6 BCL5A, LAZ3, ZBTB27, ZNF51, BCL6 BCL6 transcription repressor B-cell lymphoma 6 protein|B cell CLL/lymphoma 6|B-cell lymphoma 5 protein|B-cell lymphoma 6 protein transcript|BCL-5|BCL-6|cys-his2 zinc finger transcription factor|lymphoma-associated zinc finger gene on chromosome 3|protein LAZ-3|zinc finger and BTB domain-containing protein 27|zinc finger protein 51|zinc finger transcription factor BCL6S
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BCL6, check out the BCL6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BCL6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Bcl-6 Antibody (A00142)
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No publications found for A00142
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Bcl-6 Antibody
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skeletal muscle using anti-Bcl-6 antibody A00142. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-20
Question
My question regarding product A00142, anti-Bcl-6 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00142 anti-Bcl-6 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-09
Question
Is this A00142 anti-Bcl-6 antibody reactive to the isotypes of BCL6?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
The immunogen of A00142 anti-Bcl-6 antibody is Synthesized peptide derived from the Internal region of human Bcl-6. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-14
Question
We are currently using anti-Bcl-6 antibody A00142 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
The anti-Bcl-6 antibody (A00142) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-17
Question
Does A00142 anti-Bcl-6 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
It shows on the product datasheet, A00142 anti-Bcl-6 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-07
Question
We have been able to see staining in rat cervix carcinoma. Are there any suggestions? Is anti-Bcl-6 antibody supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
According to literature cervix carcinoma does express BCL6. According to Uniprot.org, BCL6 is expressed in urinary bladder, skeletal muscle, liver, cervix carcinoma, among other tissues. Regarding which tissues have BCL6 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 23186163
Liver, Pubmed ID: 8274740, 8506375
Skeletal muscle, Pubmed ID: 8220427
Boster Scientific Support
Answered: 2019-09-17
Question
I am interested in to test anti-Bcl-6 antibody A00142 on human skeletal muscle for research purposes, then I may be interested in using anti-Bcl-6 antibody A00142 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
The products we sell, including anti-Bcl-6 antibody A00142, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-17
Question
Is there a BSA free version of anti-Bcl-6 antibody A00142 available?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Bcl-6 antibody A00142 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-11
Question
My colleagues were satisfied with the WB result of your anti-Bcl-6 antibody. However we have been able to see positive staining in liver nucleus using this antibody. Is that expected? Could you tell me where is BCL6 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
From what I have seen in literature, liver does express BCL6. Generally BCL6 expresses in nucleus. Regarding which tissues have BCL6 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 23186163
Liver, Pubmed ID: 8274740, 8506375
Skeletal muscle, Pubmed ID: 8220427
Boster Scientific Support
Answered: 2019-05-16
Question
Our lab used your anti-Bcl-6 antibody for WB on cervix carcinoma in a previous project. I am using human, and We want to use the antibody for IHC next. Our lab want to know about examining cervix carcinoma as well as skeletal muscle in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2018-12-12
Answer
I have checked the website and datasheets of our anti-Bcl-6 antibody and I see that A00142 has been validated on human in both WB and IHC. Thus A00142 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-12-12
Question
We are interested in using your anti-Bcl-6 antibody for positive regulation of regulatory t cell differentiation studies. Has this antibody been tested with western blotting on 293 cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-11-23
Answer
I appreciate your inquiry. This A00142 anti-Bcl-6 antibody is validated on 293 cells. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-11-23
Question
I was wanting to use your anti-Bcl-6 antibody for IHC for human skeletal muscle on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human skeletal muscle identification?
Verified Customer
Verified customer
Asked: 2018-08-14
Answer
It shows on the product datasheet, A00142 anti-Bcl-6 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human skeletal muscle in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-14
Question
Does anti-Bcl-6 antibody A00142 work for IHC with skeletal muscle?
Verified Customer
Verified customer
Asked: 2018-04-17
Answer
According to the expression profile of skeletal muscle, BCL6 is highly expressed in skeletal muscle. So, it is likely that anti-Bcl-6 antibody A00142 will work for IHC with skeletal muscle.
Boster Scientific Support
Answered: 2018-04-17
Question
Is a blocking peptide available for product anti-Bcl-6 antibody (A00142)?
F. Mitchell
Verified customer
Asked: 2014-07-23
Answer
We do provide the blocking peptide for product anti-Bcl-6 antibody (A00142). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-07-23
Question
I have attached the WB image, lot number and protocol we used for skeletal muscle using anti-Bcl-6 antibody A00142. Please let me know if you require anything else.
L. Zhang
Verified customer
Asked: 2014-01-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-01-22
Question
I see that the anti-Bcl-6 antibody A00142 works with IHC, what is the protocol used to produce the result images on the product page?
B. Carter
Verified customer
Asked: 2013-05-06
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-05-06
