Click image to see more details
Product Info Summary
| SKU: | PB9195 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-FOXO1A Antibody Picoband®
SKU/Catalog Number
PB9195
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FOXO1A Antibody Picoband® catalog # PB9195. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FOXO1A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9195)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human FOXO1A recombinant protein (Position: N456-G655). Human FOXO1A shares 93% amino acid (aa) sequence identity with both mouse and rat FOXO1A.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9195 is reactive to FOXO1 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
70 kDa
Calculated molecular weight
69662 MW
Background of FOXO1
Forkhead box protein O1, also known as FKHR or FOXO1A, is a protein that in humans is encoded by the FOXO1 gene. It is mapped to 13q14.11. This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. FOXO1 plays important roles in regulation of gluconeogenesis and glycogenolysis by insulin signaling, and is also central to the decision for a preadipocyte to commit to adipogenesis. This gene is primarily regulated through phosphorylation on multiple residues. Its transcriptional activity is dependent on its phosphorylation state. What's more, FOXO1 may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9195 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Rat, Human
Positive Control
WB: rat lung tissue, rat brain tissue, human COLO320 whole cell, human HepG2 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of FOXO1A using anti-FOXO1A antibody (PB9195).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human FOXO1A protein 0.5 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXO1A antigen affinity purified polyclonal antibody (Catalog # PB9195) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXO1A at approximately 39 kDa. The expected band size for FOXO1A is at 39 kDa.
Click image to see more details
Figure 2. Western blot analysis of FOXO1A using anti-FOXO1A antibody (PB9195).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: rat brain tissue lysates,
Lane 3: human COLO320 whole cell lysates,
Lane 4: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXO1A antigen affinity purified polyclonal antibody (Catalog # PB9195) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXO1A at approximately 70 kDa. The expected band size for FOXO1A is at 70 kDa.
Protein Target Info & Infographic
Gene/Protein Information For FOXO1 (Source: Uniprot.org, NCBI)
Gene Name
FOXO1
Full Name
Forkhead box protein O1
Weight
69662 MW
Alternative Names
Forkhead box protein O1;Forkhead box protein O1A;Forkhead in rhabdomyosarcoma;FOXO1;FKHR, FOXO1A; FOXO1 FKH1, FKHRA, FOXO1 forkhead box O1 forkhead box protein O1|forkhead box protein O1A|forkhead, Drosophila, homolog of, in rhabdomyosarcoma
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FOXO1, check out the FOXO1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FOXO1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FOXO1A Antibody Picoband® (PB9195)
Hello CJ!
PB9195 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Prolonged overnutrition with fructose or fat induces metabolic derangements in rats by disrupting the crosstalk between the hypothalamus and periphery: Possible amelioration with fenofibrate
Dong P, Mai Y, Zhang Z, Mi L, Wu G, Chu G, Yang G, Sun S. Acta Biochim Biophys Sin (Shanghai). 2014 Jul;46(7):565-71. Doi: 10.1093/Abbs/Gmu043. Epub 2014 May 26. Mir-15A/B Promote Adipogenesis In Porcine Pre-Adipocyte Via Repressing Foxo1.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-FOXO1A Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-FOXO1A Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
16 Customer Q&As for Anti-FOXO1A Antibody Picoband®
Question
We are currently using anti-FOXO1A antibody PB9195 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on bovine tissues as well?
O. Wu
Verified customer
Asked: 2020-04-30
Answer
The anti-FOXO1A antibody (PB9195) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-30
Question
Do you have a BSA free version of anti-FOXO1A antibody PB9195 available?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-FOXO1A antibody PB9195 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-22
Question
We have observed staining in human cervix carcinoma. Are there any suggestions? Is anti-FOXO1A antibody supposed to stain cervix carcinoma positively?
Z. Collins
Verified customer
Asked: 2020-04-21
Answer
From literature cervix carcinoma does express FOXO1. From Uniprot.org, FOXO1 is expressed in female gonad, rhabdomyosarcoma, lymph placenta, cervix carcinoma, among other tissues. Regarding which tissues have FOXO1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231, 23186163
Lymph, and Placenta, Pubmed ID: 15489334
Rhabdomyosarcoma, Pubmed ID: 9479491
Boster Scientific Support
Answered: 2020-04-21
Question
I have attached the WB image, lot number and protocol we used for lymph placenta using anti-FOXO1A antibody PB9195. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-19
Question
I see that the anti-FOXO1A antibody PB9195 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-17
Question
I was wanting to use your anti-FOXO1A antibody for WB for human lymph placenta on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human lymph placenta identification?
Z. Patel
Verified customer
Asked: 2019-09-12
Answer
It shows on the product datasheet, PB9195 anti-FOXO1A antibody has been tested for WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human lymph placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-12
Question
Would PB9195 anti-FOXO1A antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
You can see on the product datasheet, PB9195 anti-FOXO1A antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-21
Question
Is a blocking peptide available for product anti-FOXO1A antibody (PB9195)?
R. Anderson
Verified customer
Asked: 2019-08-19
Answer
We do provide the blocking peptide for product anti-FOXO1A antibody (PB9195). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-19
Question
My lab would like using your anti-FOXO1A antibody for positive regulation of gluconeogenesis studies. Has this antibody been tested with western blotting on brain tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
Thank you for your inquiry. This PB9195 anti-FOXO1A antibody is validated on rat lung tissue, tissue lysate, brain tissue, colo320 whole cell lysate, hepg2 whole cell lysate. It is guaranteed to work for WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-07-11
Question
Can you help my question with product PB9195, anti-FOXO1A antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9195 anti-FOXO1A antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-17
Question
Would anti-FOXO1A antibody PB9195 work on zebrafish WB with lymph placenta?
A. Gonzalez
Verified customer
Asked: 2018-06-11
Answer
Our lab technicians have not tested anti-FOXO1A antibody PB9195 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-FOXO1A antibody PB9195 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish lymph placenta in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-06-11
Question
Does anti-FOXO1A antibody PB9195 work for WB with lymph placenta?
T. Taylor
Verified customer
Asked: 2018-04-26
Answer
According to the expression profile of lymph placenta, FOXO1 is highly expressed in lymph placenta. So, it is likely that anti-FOXO1A antibody PB9195 will work for WB with lymph placenta.
Boster Scientific Support
Answered: 2018-04-26
Question
Is this PB9195 anti-FOXO1A antibody reactive to the isotypes of FOXO1?
Verified Customer
Verified customer
Asked: 2018-04-09
Answer
The immunogen of PB9195 anti-FOXO1A antibody is E.coli-derived human FOXO1A recombinant protein (Position: N456-G655). Human FOXO1A shares 93% amino acid (aa) sequence identity with both mouse and rat FOXO1A. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-04-09
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymph placenta using anti-FOXO1A antibody PB9195. Let me know if you need anything else.
N. Jha
Verified customer
Asked: 2017-11-16
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-16
Question
My boss were happy with the WB result of your anti-FOXO1A antibody. However we have observed positive staining in rhabdomyosarcoma cytoplasm. using this antibody. Is that expected? Could you tell me where is FOXO1 supposed to be expressed?
T. Thomas
Verified customer
Asked: 2015-10-14
Answer
From what I have seen in literature, rhabdomyosarcoma does express FOXO1. Generally FOXO1 expresses in cytoplasm. Regarding which tissues have FOXO1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231, 23186163
Lymph, and Placenta, Pubmed ID: 15489334
Rhabdomyosarcoma, Pubmed ID: 9479491
Boster Scientific Support
Answered: 2015-10-14
Question
I am interested in to test anti-FOXO1A antibody PB9195 on human lymph placenta for research purposes, then I may be interested in using anti-FOXO1A antibody PB9195 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Krishna
Verified customer
Asked: 2013-08-13
Answer
The products we sell, including anti-FOXO1A antibody PB9195, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-08-13
