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Product Info Summary
| SKU: | PA1993 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 Antibody Picoband®
View all Pyruvate Dehydrogenase Kinase 1/PDK1 Antibodies
SKU/Catalog Number
PA1993
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 Antibody catalog # PA1993. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1993)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Mitochondrial Pyruvate dehydrogenase kinase 1, different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1993 is reactive to PDK1 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
85-91 kDa
Calculated molecular weight
49244 MW
Background of Pyruvate Dehydrogenase Kinase 1/PDK1
PDK1 (Pyruvate Dehydrogenase Kinase Isoenzyme 1), is an enzyme that in humans is encoded by the PDK1 gene. To find human PDKs, Gudi et al. (1995) used oligonucleotide primers to screen a human liver cDNA library by PCR. They identified and reported the deduced amino acid sequences of PDK1, PDK2, and PDK3. The human PDK1 gene encodes a protein with a predicted molecular mass of 49,244 Da that shares 93% identity with that of the rat PDK1 gene. Northern blot analysis showed that the PDK1 message was expressed predominantly in the heart.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1993 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: Rat Heart Tissue, Rat Skeletal Muscle Tissue, HELA Cell, M231 Cell, COLO320 Cell, SW620 Cell
IHC: Human Prostatic Cancer Tissue
Validation Images & Assay Conditions
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Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody, PA1993, Western blotting
Lane 1: Rat Heart Tissue Lysate
Lane 2: Rat Skeletal Muscle Tissue Lysate
Lane 3: HELA Cell Lysate
Lane 4: M231 Cell Lysate
Lane 5: COLO320 Cell Lysate
Lane 6: SW620 Cell Lysate
Click image to see more details
Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody, PA1993, IHC(P)
IHC(P): Human Prostatic Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For PDK1 (Source: Uniprot.org, NCBI)
Gene Name
PDK1
Full Name
[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial
Weight
49244 MW
Superfamily
PDK/BCKDK protein kinase family
Alternative Names
[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial;2.7.11.2;Pyruvate dehydrogenase kinase isoform 1;PDH kinase 1;PDK1;PDHK1; PDK1 pyruvate dehydrogenase kinase 1 [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial|pyruvate dehydrogenase (acetyl-transferring) kinase isozyme 1, mitochondrial|PDH kinase 1|mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1|pyruvate dehydrogenase kinase, isoenzyme 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PDK1, check out the PDK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PDK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 Antibody Picoband® (PA1993)
Hello CJ!
PA1993 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
lncRNA Ftx promotes aerobic glycolysis and tumor progression through the PPAR? pathway in hepatocellular carcinoma
Xu K, Ding Q, Fang Z, Zheng J, Gao P, Lu Y, Zhang Y. Cancer Gene Ther. 2010 Mar;17(3):212-22. Doi: 10.1038/Cgt.2009.66. Epub 2009 Oct 9. Silencing Of Hif-1Alpha Suppresses Tumorigenicity Of Renal Cell Carcinoma Through Induction Of Apoptosis.
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 Antibody Picoband®
Question
I have a question about product PA1993, anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1993 anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-07
Question
I was wanting to use your anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 antibody for IHC for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?
N. Parker
Verified customer
Asked: 2019-10-01
Answer
As indicated on the product datasheet, PA1993 anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 antibody has been tested for IHC, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-01
Question
I would like to test anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 antibody PA1993 on human liver for research purposes, then I may be interested in using anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 antibody PA1993 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
The products we sell, including anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 antibody PA1993, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-09
Question
Is a blocking peptide available for product anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 antibody (PA1993)?
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
We do provide the blocking peptide for product anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 antibody (PA1993). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-02
Question
We are currently using anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 antibody PA1993 for rat tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on bovine tissues as well?
B. Jha
Verified customer
Asked: 2016-08-01
Answer
The anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 antibody (PA1993) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-08-01
Question
Is this PA1993 anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 antibody reactive to the isotypes of PDK1?
A. Kulkarni
Verified customer
Asked: 2016-04-15
Answer
The immunogen of PA1993 anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Mitochondrial Pyruvate dehydrogenase kinase 1(408-421aa WKHYNTNHEADDWC), different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-04-15
