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Product Info Summary
| SKU: | A04942-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Foxj1 (HFH4) Antibody
View all FoxJ1/HFH4 Antibodies
SKU/Catalog Number
A04942-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Foxj1 (HFH4) Antibody (Catalog # A04942-1). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Foxj1 (HFH4) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04942-1)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.413~417( D-W-A-S-V) derived from Human Foxj1 (HFH4).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04942-1 is reactive to FOXJ1 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
68560 MW
Background of FoxJ1/HFH4
May play an important role in cell fate determination during lung development and in spermatogenesis.
Li C.-S., Chae S.-C., Lee J.-H., Zhang Q., Chung H.-T.J. Hum. Genet. 51:292-297(2006)
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04942-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 58kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:50~1:200
Immunohistochemistry: 1:50~1:200
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of FOXJ1 using anti-FOXJ1 antibody (A04942-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXJ1 antigen affinity purified polyclonal antibody (Catalog # A04942-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for FOXJ1.
Protein Target Info & Infographic
Gene/Protein Information For FOXJ1 (Source: Uniprot.org, NCBI)
Gene Name
FOXJ1
Full Name
Forkhead box protein J1
Weight
68560 MW
Superfamily
FOXJ1 family
Alternative Names
Complement component C1q receptor;C1q/MBL/SPA receptor;C1qR;C1qR (p);C1qRp;CDw93;Complement component 1 q subcomponent receptor 1;Matrix-remodeling-associated protein 4;CD93;CD93;C1QR1, MXRA4; FOXJ1 CILD43, FKHL13, HFH-4, HFH4 forkhead box J1 forkhead box protein J1|fork head homologue 4|forkhead transcription factor HFH-4|forkhead-like 13|forkhead-related protein FKHL13|hepatocyte nuclear factor 3 forkhead homolog 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FOXJ1, check out the FOXJ1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FOXJ1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Foxj1 (HFH4) Antibody (A04942-1)
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No publications found for A04942-1
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-Foxj1 (HFH4) Antibody
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal lung using anti-Foxj1 (HFH4) antibody A04942-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-28
Question
Is a blocking peptide available for product anti-Foxj1 (HFH4) antibody (A04942-1)?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
We do provide the blocking peptide for product anti-Foxj1 (HFH4) antibody (A04942-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-14
Question
you antibody to test anti-Foxj1 (HFH4) antibody A04942-1 on human fetal lung for research purposes, then I may be interested in using anti-Foxj1 (HFH4) antibody A04942-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
The products we sell, including anti-Foxj1 (HFH4) antibody A04942-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-18
Question
Is there a BSA free version of anti-Foxj1 (HFH4) antibody A04942-1 available?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Foxj1 (HFH4) antibody A04942-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-05
Question
My question regarding product A04942-1, anti-Foxj1 (HFH4) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04942-1 anti-Foxj1 (HFH4) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-17
Question
Would anti-Foxj1 (HFH4) antibody A04942-1 work on canine WB with fetal lung?
M. Zhao
Verified customer
Asked: 2018-11-01
Answer
Our lab technicians have not validated anti-Foxj1 (HFH4) antibody A04942-1 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Foxj1 (HFH4) antibody A04942-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine fetal lung in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-11-01
Question
We are currently using anti-Foxj1 (HFH4) antibody A04942-1 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-04-16
Answer
The anti-Foxj1 (HFH4) antibody (A04942-1) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-04-16
Question
I see that the anti-Foxj1 (HFH4) antibody A04942-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-04-10
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-04-10
Question
Does anti-Foxj1 (HFH4) antibody A04942-1 work for WB with fetal lung?
Verified Customer
Verified customer
Asked: 2017-12-25
Answer
According to the expression profile of fetal lung, FOXJ1 is highly expressed in fetal lung. So, it is likely that anti-Foxj1 (HFH4) antibody A04942-1 will work for WB with fetal lung.
Boster Scientific Support
Answered: 2017-12-25
Question
Is this A04942-1 anti-Foxj1 (HFH4) antibody reactive to the isotypes of FOXJ1?
J. Moore
Verified customer
Asked: 2016-11-23
Answer
The immunogen of A04942-1 anti-Foxj1 (HFH4) antibody is Peptide sequence around aa.413~417 ( D-W-A-S-V) derived from Human Foxj1 (HFH4) . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-11-23
Question
I was wanting to use your anti-Foxj1 (HFH4) antibody for WB for human fetal lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human fetal lung identification?
S. Krishna
Verified customer
Asked: 2015-11-16
Answer
You can see on the product datasheet, A04942-1 anti-Foxj1 (HFH4) antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human fetal lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-11-16
Question
I have attached the WB image, lot number and protocol we used for fetal lung using anti-Foxj1 (HFH4) antibody A04942-1. Please let me know if you require anything else.
M. Mangal
Verified customer
Asked: 2014-03-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-03-25
Question
Will A04942-1 anti-Foxj1 (HFH4) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Moore
Verified customer
Asked: 2013-10-04
Answer
You can see on the product datasheet, A04942-1 anti-Foxj1 (HFH4) antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-10-04
