Forkhead box protein J1 Antibodies

Forkhead box protein J1 (FOXJ1)

Transcription factor specifically required for the formation of motile cilia. Acts by activating transcription of genes that mediate assembly of motile cilia, for example CFAP157.

Binds the DNA consensus sequences 5'-HWDTGTTTGTTTA-3' or 5'- KTTTGTTGTTKTW-3' (where H is not G, W is A or T, D is not C, and K is G or T). Activates the transcription of many different ciliary proteins in the developing lung and brain.

Gene Information

Human
Gene Name:	FOXJ1
Uniprot:	Q92949
Entrez:	2302

Family

Belongs to:
FOXJ1 family

Alternative Names

FKHL13; FKHL13forkhead transcription factor HFH-4; forkhead box J1; forkhead-like 13; Forkhead-related protein FKHL13; FoxJ1; Hepatocyte nuclear factor 3 forkhead homolog 4; HFH-4; HFH4fork head homologue 4; HFH-4forkhead box protein J1; MGC35202

Molecular Weight

Mass (kDA):
45.247 kDA

Genomic Context

Human
Location:	17q25.1
Sequence:	17; NC_000017.11 (76136333..76141245, complement)

Tissue Specificity

Testis, oviduct, lung and brain cortex.

Subcellular Localizations

Nucleus.

Diseases

• Lung Diseases
• Autoimmunity
• Malignant Neoplasms
• Neoplasms
• Tissue Adhesions
• Hyperplasia
• Lupus Erythematosus, Systemic
• Chronic Obstructive Airway Disease
• Asthma
• Metaplasia
• Lung Diseases, Obstructive
• Inflammation
• Mucinous Adenocarcinoma

• Cystic Fibrosis
• Kartagener Syndrome
• Situs Inversus
• Respiration Disorders
• Fibrosis
• Cytomegalovirus Infections
• Autoimmune Diseases

Interacting Proteins

• RFX2

Pathways

• Cell Differentiation
• Localization
• Cell Proliferation
• Epithelial Cell Differentiation
• Lung Development
• Lung Morphogenesis
• Transport
• Cell Adhesion
• Cell Activation
• Regeneration
• Pathogenesis
• Cell Cycle
• Secretion

• T Cell Activation
• Intraflagellar Transport
• Epithelial Cell Proliferation
• Cytokine Production
• Developmental Process
• Neurogenesis
• T Cell Proliferation

PTMs

• Phosphorylation
• Demethylation

• Cleavage
• Acetylation

• Methylation

Research Areas

• Cancer
• Transcription Factors and Regulators

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/FOXJ1

Best Uses of the FOXJ1 Marker

If you've recently purchased a FOXJ1 marker, you might be thinking about what it is and how it works. There are many benefits and applications for this marker, making it a great option for DNA-based PCR amplification. Here's a brief overview. Here's a brief review of the purpose and how it can aid your research. This article will provide the information you need to determine the appropriate marker for your project.

Function

Researchers have identified a crucial regulator of the motile multiciliated cells differentiation program of human airway epithelium. It is the FOXJ1 gene. This gene also regulates differentiation of multiciliated and basal cells. Thus, if you are trying to stop the development of asthma, FOXJ1 might be useful. Additionally, this gene has important roles in cilia.

The gene that encodes FOXJ1 is a member of the Forkhead/winged-helix family of transcription factors. The 100 amino acid DNA-binding domain has been implicated in regulating several aspects of immune system function. Foxj1 plays an important role in the development of cilia, the respiratory system, as well as the reproductive system. Mutations in the FOXJ1 gene can lead to abnormal cilia within tissues, including the respiratory and reproductive system. Mutations in this gene also cause suppressed T cell activity.

Western blots are used to study the expression levels of FOXJ1 genes in different tissues. In normal tissue, the amount of FOXJ1 was low. This level increased for 12 hours after TBI and reached its peak on day 3. After three days, the expression of the FOXJ1 gene declined slowly, but it was still peaking at day 28. The gene is also expressed in multiple tissues including the heart and intestine.

The promoter of FOXJ1 can be found in cells that are ciliated in the lung airways, choroidplexus and ependyma. It is also found in the oviduct. It is also expressed in testis and sperm and is involved in epithelial repair. It also plays a key role in organ development. The gene also regulates transcription of genes that are associated with the cilia.

The cilia in fish are directly linked to the kinocilia in amniotes. Although this mechanism might not be applicable to mammals, knowing how cilia regulate the development of the ear might have significant mechanistic implications for the development of the ear in mammals. Therefore, the FOXJ1 gene plays a major role in the study of cilia. However, it is not clear whether the FOXJ1 gene in humans plays a part in the formation of cilia.

Amplification of PCR

This study investigated the effects of PCR amplifying with FOXj1 marker on FOXJ1 localization in NPs with neutrophils as well and non-neutrophilic ones. The scores for localization of FOXJ1 were higher in patients with severe NPs in comparison to those with less severe NPs. This marker was also related to other comorbidities like asthma and COPD. However the marker does not differentiate between non-inflammatory and inflammatory NPs.

For PCR amplifying the FOXj1 marker, an genomic DNA fragment that is corresponding to the 5' flanking sequence of human FOXJ1 was amplified. This sequence was extracted from Genbank sequence AC018665. This fragment was digested with SalI and EcoRI, and then exchanged with CMV enhancer and b-actin promor and pCAGG. M. Reth's laboratory gathered FOXJ1 human FOXJ1 bglobin polyA.

The development of stationary and motile cilia, in mice, and other vertebrates is controlled by the FOXJ1 gene. A similar system is needed for ciliated epithelial cells of the airway. Many mouse lines were developed by using the FOXJ1 promoter driving Cre Recombinase and analyzed for their functions. Once the gene has been successfully removed, it can be identified using a cell-based approach.

AR has been linked to allergic nasal mucosa by FOXJ1 expression in the top airway cilia. This gene is responsible for the development of mucosas that are motile and allergic-specific cilia. There is a strong correlation between the severity the disease and an aberrant FOXJ1 location. In this regard, the FOXJ1 gene could be involved in the development of NP.

The study was conducted using wild-type mice and FoxJ1/ mice. Both genetic models indicated that the expression of FOXJ1 was comparable between the two sexes. Additionally, the Dnaic1-mice had a similar developmental time-course. They showed progressive growth in ventricular volume by P21. To assess the SCN cell type in the hemispheres, mice were perfused between P6 and at P21. They were then fixed by paraformaldehyde in pH 7.4.

Applications

Recently recently, the FOXJ1 marker was discovered to be an entirely new variant. The FOXJ1 gene is located on the human chromosome 17q25.1, and its paralog, FOXK2, is related to Embryonic and induced pluripotent Stem Cells, Lineage-specific Markers, and DNA-binding transcription factor activity. There are a variety of applications for the FOXJ1 gene.

To perform the SP immunohistochemistry, the anti-human FOXJ1 monoclonal antibody was used. Reagents were purchased from Abcam. The molecular basis of this gene isn't fully understood, however future research is needed to determine whether FOXJ1 is required to distinguish between goblet cells and ciliated cells. Further research is required to better understand the role of the FOXJ1 gene in the airway epithelium.

A number of studies have shown that there is evidence that FOXJ1 protein is expressed in various types of tumors, with the level of expression varying based on the stage of the tumor. However, it is unknown whether FOXJ1 has an inhibitory or a promotor function in the development of tumors. This study examined the expression of FOXJ1 in bladder epithelial cancer to determine the relationship between the stage of cancer and FOXJ1 expression.

FOXJ1 is a member of the Fox family transcription factors. It plays an important role in the process of differentiation in adult neural stem cells. This cell type is responsible for the growth of neurons, astrocytes and oligodendrocytes. Recent studies suggest that FOXJ1 may be a tumor suppressor gene. In one study, FOXJ1 inhibited the activity of the nuclear factor NF-kB. The activity of NFKB is associated with the formation of tumors. Additionally, it stimulates the production of anti-apoptotic as well as chemotactic proteins. FOXJ1 also inhibits NANOG (a transcription factor expressed within stem cells).

Alongside transcriptional regulation FOXJ1 is also involved in the expression of other genes. For instance, NKX2-1 mRNA enhances FOXJ1 expression in differentiated NHBE tissues. After ALI cultivation the MUC5AC mRNA is expressed in undifferentiated HBE cell lines.

Benefits

The stem cell potential and ciliogenesis of adult human cells depend on the activity of FoxJ1. Although its precise role in adult development remains unknown however, it is believed that the gene plays a key part in the regeneration of the spinal cord after SCI. The benefits of the FOXJ1 marker in assessing spinal cord health are numerous. We will now look at some of these benefits. Hopefully, the new knowledge will help researchers understand the role played by FOXJ1 in human disease.

FoxJ1 expression is crucial for the differentiation of neural cells including the glial cells. The ability of neural cells and glial cells differentiate into neurospheres can be impaired without FoxJ1. However, FoxJ1-KI-CreERT2-TdTomato mice still show normal ependymal self-renewal and migration capacity. This study further demonstrates that FOXJ1 is crucial for stem cell function in adult human spinal cord.

Researchers can make use of the FOXJ1 marker to provide important data for their cancer research. The premature exit of the proliferative stage due to the loss of this gene results in more neuronal differentiation. It also helps in the study and understanding of cell biology which will help us better discover the causes of various diseases. However the advantages of this marker aren't yet fully realized. This marker is used to develop spinal cord tissues. However it could also be a useful tool in the near future.

The number of cells expressing EGFP in the FOXJ1CreERT2-YFP model was similar to that observed in mouse cancer models. In a separate study, the mice harboring the FOXJ1-KI-CreERT2-TdTomato were genetically corrected to express the gene. In mice expressing the FOXJ1-CreERT2-YFP reporter, EGFP+ cells were located in the white matter of the lateral.

In addition, FoxJ1 is an essential gene in the development of brains. Eliminating this gene in mice results in a drastic decrease in the number of reporter-positive cells in the central canal. In addition, FoxJ1-KI-CreERT2-TdTomato cells showed an increased expression of Pax2 while expressing no astrocytic markers. FoxJ1 is therefore a crucial gene that is involved in brain development as well as neurogenesis. It also has other functions.