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Product Info Summary
| SKU: | A01552-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-BIM(Ab-69) BCL2L11 Antibody
SKU/Catalog Number
A01552-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-BIM(Ab-69) BCL2L11 Antibody (Catalog # A01552-1). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BIM(Ab-69) BCL2L11 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01552-1)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.67~71 (P-A-S-P-G) derived from Human BIM.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01552-1 is reactive to BCL2L11 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
22171 MW
Background of BCL2L11
Induces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-a1, isoform Bim-a2 and isoform Bim-a3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis.
Fukazawa H, et al. (2004) Mol Cancer Ther ; 3(10): 1281-1288
Luciano F, et al. (2003) Oncogene ; 22(43): 6785-6793
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01552-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 23kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts from Hela cells using BIM(Ab-69) antibody A01552-1.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BIM(Ab-69) antibody A01552-1(left) or the same antibody preincubated with blocking peptide(right).
Protein Target Info & Infographic
Gene/Protein Information For BCL2L11 (Source: Uniprot.org, NCBI)
Gene Name
BCL2L11
Full Name
Bcl-2-like protein 11
Weight
22171 MW
Superfamily
Bcl-2 family
Alternative Names
Bcl-2-like protein 11;Bcl2-L-11;Bcl2-interacting mediator of cell death;BCL2L11;BIM; BCL2L11 BAM, BIM, BOD BCL2 like 11 bcl-2-like protein 11|BCL2-like 11 (apoptosis facilitator)|bcl-2 interacting mediator of cell death|bcl-2 interacting protein Bim|bcl-2-related ovarian death agonist
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BCL2L11, check out the BCL2L11 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BCL2L11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BIM(Ab-69) BCL2L11 Antibody (A01552-1)
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No publications found for A01552-1
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-BIM(Ab-69) BCL2L11 Antibody
Question
Is a blocking peptide available for product anti-BIM(Ab-69) antibody (A01552-1)?
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
We do provide the blocking peptide for product anti-BIM(Ab-69) antibody (A01552-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-28
Question
I see that the anti-BIM(Ab-69) antibody A01552-1 works with IHC, what is the protocol used to produce the result images on the product page?
J. Carter
Verified customer
Asked: 2019-08-28
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-28
Question
Will A01552-1 anti-BIM(Ab-69) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Evans
Verified customer
Asked: 2019-02-22
Answer
As indicated on the product datasheet, A01552-1 anti-BIM(Ab-69) antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-02-22
Question
Will anti-BIM(Ab-69) antibody A01552-1 work for IHC with peripheral blood spleen?
K. Lewis
Verified customer
Asked: 2017-10-04
Answer
According to the expression profile of peripheral blood spleen, BCL2L11 is highly expressed in peripheral blood spleen. So, it is likely that anti-BIM(Ab-69) antibody A01552-1 will work for IHC with peripheral blood spleen.
Boster Scientific Support
Answered: 2017-10-04
Question
I was wanting to use your anti-BIM(Ab-69) antibody for IHC for mouse peripheral blood spleen on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse peripheral blood spleen identification?
R. Williams
Verified customer
Asked: 2016-08-12
Answer
As indicated on the product datasheet, A01552-1 anti-BIM(Ab-69) antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse peripheral blood spleen in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-08-12
