Anti-FOXA1 Antibody Picoband™

Product Name

Anti-FOXA1 Antibody Picoband™

View all HNF-3 alpha/FoxA1 Antibodies

SKU/Catalog Number

A00266-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-FOXA1 Antibody Picoband™ catalog # A00266-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-FOXA1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00266-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human FOXA1 recombinant protein (Position: M1-T55).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00266-1 is reactive to FOXA1 in Human, Mouse, Rat

Applications

A00266-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

49 kDa

Calculated molecular weight

68762 MW

Background of HNF-3 alpha/FoxA1

Forkhead box protein A1 (FOXA1), also known as hepatocyte nuclear factor 3-alpha (HNF-3A), is a proteinthat in humans is encoded by the FOXA1 gene. This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of FOXA1 using anti-FOXA1 antibody (A00266-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human MCF-7 cell lysate,
Lane 2: human A549 cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXA1 antigen affinity purified polyclonal antibody (Catalog # A00266-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXA1 at approximately 49KD. The expected band size for FOXA1 is at 49KD.

Gene/Protein Information For FOXA1 (Source: Uniprot.org, NCBI)

Alternative Names

forkhead box A1; Forkhead box protein A1; FoxA1; hepatocyte nuclear factor 3, alpha; hepatocyte nuclear factor 3-alpha; HNF3 alpha; HNF-3 alpha; HNF-3A; HNF-3-alpha; HNF3ATCF-3A; MGC33105; TCF3A; Transcription factor 3A FOXA1 HNF3A, TCF3A forkhead box A1 hepatocyte nuclear factor 3-alpha|HNF-3-alpha|HNF-3A|TCF-3A|forkhead box protein A1|transcription factor 3A

For more info on FOXA1, check out the FOXA1 Infographic

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In this infographic, you will see the following information for FOXA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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