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Product Info Summary
| SKU: | A00266-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-FOXA1 Antibody Picoband®
View all HNF-3 alpha/FoxA1 Antibodies
SKU/Catalog Number
A00266-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FOXA1 Antibody Picoband® catalog # A00266-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FOXA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00266-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human FOXA1 recombinant protein (Position: M1-T55).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00266-1 is reactive to FOXA1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
49 kDa
Calculated molecular weight
68762 MW
Background of HNF-3 alpha/FoxA1
Forkhead box protein A1 (FOXA1), also known as hepatocyte nuclear factor 3-alpha (HNF-3A), is a proteinthat in humans is encoded by the FOXA1 gene. This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00266-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human MCF-7 cell, human A549 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of FOXA1 using anti-FOXA1 antibody (A00266-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human MCF-7 cell lysate,
Lane 2: human A549 cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXA1 antigen affinity purified polyclonal antibody (Catalog # A00266-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXA1 at approximately 49KD. The expected band size for FOXA1 is at 49KD.
Protein Target Info & Infographic
Gene/Protein Information For FOXA1 (Source: Uniprot.org, NCBI)
Gene Name
FOXA1
Full Name
Hepatocyte nuclear factor 3-alpha
Weight
68762 MW
Alternative Names
Hepatocyte nuclear factor 3-alpha; HNF-3-alpha; HNF-3A; Forkhead box protein A1; Transcription factor 3A; TCF-3A; FOXA1; HNF3A; TCF3A FOXA1 HNF3A, TCF3A forkhead box A1 hepatocyte nuclear factor 3-alpha|HNF-3-alpha|HNF-3A|TCF-3A|forkhead box protein A1|transcription factor 3A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FOXA1, check out the FOXA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FOXA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FOXA1 Antibody Picoband® (A00266-1)
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Customer Q&As
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1 Customer Q&As for Anti-FOXA1 Antibody Picoband®
Question
We are currently using anti-FOXA1 antibody A00266-1 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
The anti-FOXA1 antibody (A00266-1) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-28
