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Product Info Summary
| SKU: | PB9760 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-FMO2 Antibody Picoband®
SKU/Catalog Number
PB9760
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FMO2 Antibody Picoband® catalog # PB9760. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FMO2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9760)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human FMO2, different from the related mouse and rat sequences by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9760 is reactive to FMO2 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
54 kDa
Calculated molecular weight
53644 MW
Background of FMO2
Dimethylaniline monooxygenase [N-oxide-forming] 2 is an enzyme that in humans is encoded by the FMO2 gene. This gene encodes a flavin-containing monooxygenase family member. It is an NADPH-dependent enzyme that catalyzes the N-oxidation of some primary alkylamines through an N-hydroxylamine intermediate. However, some human populations contain an allele (FMO2*2A) with a premature stop codon, resulting in a protein that is C-terminally-truncated, has no catalytic activity, and is likely degraded rapidly. This gene is found in a cluster with other related family members on chromosome 1. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9760 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: A549 Whole Cell, HELA Whole Cell, MCF-7 Whole Cell, SW620 Whole Cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of FMO2 using anti-FMO2 antibody (PB9760).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions.
Lane 1: A549 Whole Cell Lysate,
Lane 2: HELA Whole Cell Lysate,
Lane 3: MCF-7 Whole Cell Lysate,
Lane 4: SW620 Whole Cell Lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FMO2 antigen affinity purified polyclonal antibody (Catalog # PB9760) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FMO2 at approximately 54 kDa. The expected band size for FMO2 is at 54 kDa.
Protein Target Info & Infographic
Gene/Protein Information For FMO2 (Source: Uniprot.org, NCBI)
Gene Name
FMO2
Full Name
Dimethylaniline monooxygenase [N-oxide-forming] 2
Weight
53644 MW
Superfamily
FMO family
Alternative Names
Dimethylaniline monooxygenase [N-oxide-forming] 2;1.14.13.8;Dimethylaniline oxidase 2;FMO 1B1;Pulmonary flavin-containing monooxygenase 2;FMO 2;FMO2; FMO2 FMO1B1 flavin containing dimethylaniline monoxygenase 2 dimethylaniline monooxygenase [N-oxide-forming] 2|FMO, pulmonary|dimethylaniline oxidase 2|flavin containing monooxygenase 2 (non-functional)|pulmonary flavin-containing monooxygenase 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FMO2, check out the FMO2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FMO2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FMO2 Antibody Picoband® (PB9760)
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Customer Q&As
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7 Customer Q&As for Anti-FMO2 Antibody Picoband®
Question
Do you have a BSA free version of anti-FMO2 antibody PB9760 available?
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-FMO2 antibody PB9760 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-17
Question
Is a blocking peptide available for product anti-FMO2 antibody (PB9760)?
O. Li
Verified customer
Asked: 2020-02-05
Answer
We do provide the blocking peptide for product anti-FMO2 antibody (PB9760). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-05
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skeletal muscle using anti-FMO2 antibody PB9760. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-03
Question
My question regarding product PB9760, anti-FMO2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Thomas
Verified customer
Asked: 2019-07-19
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9760 anti-FMO2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-19
Question
We are currently using anti-FMO2 antibody PB9760 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?
S. Carter
Verified customer
Asked: 2019-07-04
Answer
The anti-FMO2 antibody (PB9760) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-04
Question
Would anti-FMO2 antibody PB9760 work on canine WB with upper lobe of lung?
Verified Customer
Verified customer
Asked: 2018-11-13
Answer
Our lab technicians have not validated anti-FMO2 antibody PB9760 on canine. You can run a BLAST between canine and the immunogen sequence of anti-FMO2 antibody PB9760 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine upper lobe of lung in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-11-13
Question
Our lab want to know about to test anti-FMO2 antibody PB9760 on human skeletal muscle for research purposes, then I may be interested in using anti-FMO2 antibody PB9760 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-06-14
Answer
The products we sell, including anti-FMO2 antibody PB9760, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-06-14
