Click image to see more details
Product Info Summary
| SKU: | A14686-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Transmembrane protein 199 TMEM199 Antibody Picoband®
SKU/Catalog Number
A14686-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Transmembrane protein 199 TMEM199 Antibody catalog # A14686-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Transmembrane protein 199 TMEM199 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14686-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TMEM199 recombinant protein (Position: E20-H129).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A14686-1 is reactive to TMEM199 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
23 kDa
Calculated molecular weight
64099 MW
Background of TMEM199
TMEM199 encodes a protein homologous to the yeast V-ATPase assembly factor Vma12 and appears to be involved in Golgi homeostasis. The protein encoded by this gene has been observed to localize to the endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC) and coat protein complex I (COPI) in some human cells. Defects in this gene are a cause of congenital disorder of glycosylation, type IIp. By genomic sequence analysis, the TMEM199 gene is mapped to chromosome 17q11.1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14686-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human Hela whole cell, human placenta tissue, human Caco-2 whole cell, human T-47D whole cell, human U-87MG whole cell, human K562 whole cell, human U2OS whole cell, human PC-3 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of TMEM199 using anti-TMEM199 antibody (A14686-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human placenta tissue lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human T-47D whole cell lysates,
Lane 5: human U-87MG whole cell lysates,
Lane 6: human K562 whole cell lysates,
Lane 7: human U2OS whole cell lysates,
Lane 8: human PC-3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM199 antigen affinity purified polyclonal antibody (Catalog # A14686-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM199 at approximately 23KD. The expected band size for TMEM199 is at 23KD.
Protein Target Info & Infographic
Gene/Protein Information For TMEM199 (Source: Uniprot.org, NCBI)
Gene Name
TMEM199
Full Name
Transmembrane protein 199
Weight
64099 MW
Alternative Names
Transmembrane protein 199; TMEM199; C17orf32 TMEM199 C17orf32, CDG2P, VMA12, VPH2 transmembrane protein 199 transmembrane protein 199
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TMEM199, check out the TMEM199 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TMEM199: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Transmembrane protein 199 TMEM199 Antibody Picoband® (A14686-1)
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Customer Q&As
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5 Customer Q&As for Anti-Transmembrane protein 199 TMEM199 Antibody Picoband®
Question
We are interested in to test anti-TMEM199 antibody A14686-1 on rat heart left ventricle for research purposes, then I may be interested in using anti-TMEM199 antibody A14686-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-01-10
Answer
The products we sell, including anti-TMEM199 antibody A14686-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-01-10
Question
Is this A14686-1 anti-TMEM199 antibody reactive to the isotypes of TMEM199?
Verified Customer
Verified customer
Asked: 2018-08-06
Answer
The immunogen of A14686-1 anti-TMEM199 antibody is E.coli-derived human TMEM199 recombinant protein (Position: E20-H129). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-08-06
Question
I see that the anti-TMEM199 antibody A14686-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-12-14
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-12-14
Question
We are currently using anti-TMEM199 antibody A14686-1 for human tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
D. Kulkarni
Verified customer
Asked: 2017-10-06
Answer
The anti-TMEM199 antibody (A14686-1) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-06
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for heart left ventricle using anti-TMEM199 antibody A14686-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-08-01
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-08-01
