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Product Info Summary
| SKU: | M06107 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-Flotillin 2 FLOT2 Rabbit Monoclonal Antibody
View all Flotillin 2 Antibodies
SKU/Catalog Number
M06107
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Flotillin 2 FLOT2 Rabbit Monoclonal Antibody catalog # M06107. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Flotillin 2 FLOT2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06107)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GB-6
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Flotillin 2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M06107 is reactive to FLOT2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
47 kDa
Calculated molecular weight
47 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06107 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IP 1:30
Positive Control
WB: human Hela whole cell, human 293T whole cell, human RT4 whole cell, rat skin tissue, rat heart tissue, mouse skin tissue, mouse heart tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of FLOT2 using anti-FLOT2 antibody (M06107).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: rat skin tissue lysates,
Lane 5: rat heart tissue lysates,
Lane 6: mouse skin tissue lysates,
Lane 7: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FLOT2 antigen affinity purified monoclonal antibody (Catalog # M06107) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FLOT2 at approximately 47 kDa. The expected band size for FLOT2 is at 47 kDa.
Protein Target Info & Infographic
Gene/Protein Information For FLOT2 (Source: Uniprot.org, NCBI)
Gene Name
FLOT2
Full Name
Flotillin-2
Weight
47 kDa
Superfamily
band 7/mec-2 family
Alternative Names
Flotillin-2;Epidermal surface antigen;ESA;Membrane component chromosome 17 surface marker 1;FLOT2;ESA1, M17S1; FLOT2 ECS-1, ECS1, ESA, ESA1, M17S1 flotillin 2 flotillin-2|Flotillin 2 (epidermal surface 1)|epidermal surface |membrane component chromosome 17 surface marker 1|membrane component, chromosome 17, surface marker 1 (35kD protein by ECS-1)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FLOT2, check out the FLOT2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FLOT2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Flotillin 2 FLOT2 Rabbit Monoclonal Antibody (M06107)
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