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Product Info Summary
| SKU: | A03722 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-FKBP8 Antibody
SKU/Catalog Number
A03722
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-FKBP8 Antibody (Catalog # A03722). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-FKBP8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03722)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human FKBP8 protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03722 is reactive to FKBP8 in Human
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
52456 MW
Background of FKBP38
FKBP8 (also known as FK506-binding protein 38) is a member of the immunophilin family that has been implicated to play an important role in apoptosis through its involvement in the mechanism that targets Bcl-2 and Bcl-xL to the outer mitochondrial membrane (OMM). Suppression of endogenous FKBP8 by RNAi or transfection of a mutant FKBP8 missing the transmembrane domain necessary for mitochondrial insertion, resulted in the translocation of Bcl-2 and Bcl-xL from the OMM to the cytosol. It has also been suggested that FKBP8 may play a role in the regulation of apoptosis. Anti-FKBP8 Antibody is useful for researchers interested in HIV, Cancer, and Metabolism of protein research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03722 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:25,000
WB: 1:500 - 1:2,000
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of FKBP8 using anti-FKBP8 antibody (A03722).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FKBP8 antigen affinity purified polyclonal antibody (Catalog # A03722) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for FKBP8.
Protein Target Info & Infographic
Gene/Protein Information For FKBP8 (Source: Uniprot.org, NCBI)
Gene Name
FKBP8
Full Name
Peptidyl-prolyl cis-trans isomerase FKBP8
Weight
52456 MW
Alternative Names
FKBP8, FKBP 8, FKBP-38, 38 kDa FK 506 binding protein homolog, 38kDa, FK506 binding protein 8, FKBP 38 Fkbp8|38kD, 38kDa, FKBP-38, FKBP-8, FKBPR38, Fkbp3, Fkbp38, mFKBP38|FK506 binding protein 8|peptidyl-prolyl cis-trans isomerase FKBP8|38 kDa FK506-binding protein|38 kDa FKBP|FK506-binding protein 38|PPIase FKBP8|rotamase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FKBP8, check out the FKBP8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FKBP8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FKBP8 Antibody (A03722)
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No publications found for A03722
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Customer Q&As
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1 Customer Q&As for Anti-FKBP8 Antibody
Question
We are currently using anti-FKBP8 antibody A03722 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
O. Anderson
Verified customer
Asked: 2015-05-07
Answer
The anti-FKBP8 antibody (A03722) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-05-07
