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Product Info Summary
| SKU: | EK1539 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants and serum. |
Product info
Product Name
Human FOLR2/Beta Fr ELISA Kit PicoKine®
SKU/Catalog Number
EK1539
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human FOLR2/Beta Fr ELISA Kit PicoKine® (96 Tests). Quantitate Human FOLR2 in cell culture supernatants and serum. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human FOLR2/Beta Fr ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1539)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: T17-N230
Sensitivity
<1 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1539 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1539 is reactive to FOLR2 in Human samples
Validated Sample Types
cell culture supernatants and serum.
Application Guarantee
EK1539 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of FOLR2
Folate receptor beta is a protein that in humans is encoded by the FOLR2 gene. The protein encoded by this gene is a member of the folate receptor (FOLR) family, and these genes exist in a cluster on chromosome 11. Members of this gene family have a high affinity for folic acid and for several reduced folic acid derivatives, and they mediate delivery of 5-methyltetrahydrofolate to the interior of cells. This protein has a 68% and 79% sequence homology with the FOLR1 and FOLR3 proteins, respectively. Although this protein was originally thought to be specific to placenta, it can also exist in other tissues, and it may play a role in the transport of methotrexate in synovial macrophages in rheumatoid arthritis patients. Multiple transcript variants that encode the same protein have been found for this gene.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1539-CAP | Anti-Human FOLR2 Pre-coated 96-well strip microplate | 1 |
| EK1539-ST | Human FOLR2 Standard | 2 vials, 10 ng/tube |
| EK1539-DA | Human FOLR2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.028 | 0.050 | 0.101 | 0.207 | 0.456 | 0.972 | 1.941 | 2.585 |
Data Example Images
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Human FOLR2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect FOLR2, Dilution ratio of 1:5, concentration in serum is around 5.5 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human FOLR2/Beta Fr ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 342 | 1852 | 4326 | 361 | 1834 | 4010 |
| Standard deviation | 23.25 | 85.49 | 190.34 | 29.96 | 102.7 | 216.54 |
| CV (%) | 6.8% | 4.6% | 4.4% | 8.3% | 5.6% | 5.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of FOLR2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 342 | 322 | 342 | 345 | 337 | 9.17 | 2.7% |
| Sample 2 | 1852 | 1882 | 1780 | 2001 | 1878 | 79.72 | 4.2% |
| Sample 3 | 4326 | 4126 | 3772 | 4098 | 4080 | 198.63 | 4.8% |
Protein Target Info & Infographic
Gene/Protein Information For FOLR2 (Source: Uniprot.Org, NCBI)
Gene Name
FOLR2
Full Name
Folate receptor beta
Weight
29280 MW
Superfamily
folate receptor family
Alternative Names
Folate receptor beta;FR-beta;Folate receptor 2;Folate receptor, fetal/placental;Placental folate-binding protein;FBP;FOLR2; FOLR2 BETA-HFR, FBP, FBP/PL-1, FOLR1, FR-BETA, FR-P3, FRbeta folate receptor beta folate receptor beta|folate receptor 2 (fetal)|folate receptor alpha|folate receptor, fetal/placental|folate-binding protein, fetal/placental|placental folate-binding protein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on FOLR2, check out the FOLR2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FOLR2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human FOLR2/Beta Fr ELISA Kit PicoKine® (EK1539)
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No publications found for EK1539
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Customer Reviews
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Customer Q&As
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8 Customer Q&As for Human FOLR2/Beta Fr ELISA Kit PicoKine®
Question
Does EK1539 have cross-reactivity with other relevant proteins and have you tested the cross-reactivity with human FOLR1 and FOLR3?
Verified customer
Asked: 2020-10-20
Answer
For the Human FOLR2/Beta Fr ELISA Kit PicoKine™ (EK1539), our lab tested the following recombinant proteins and didn't observe any cross-reactivity: Recombinant human FOLR1, FOLR3, FOLR4 Recombinant mouse FOLR2
Boster Scientific Support
Answered: 2020-10-21
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2020-05-14
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-05-14
Question
Q: What is the optimal O.D. value for FOLR2 ELISA kit? I used your FOLR2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain FOLR2 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this FOLR2 ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of FOLR2 that can be declared/interpreted as positive by the above standard is 1pg/ml.
Boster Scientific Support
Answered: 2019-10-24
Question
Q: Can FOLR2 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
G. Edwards
Verified customer
Asked: 2019-09-28
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-09-28
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
M. Surve
Verified customer
Asked: 2019-03-22
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-03-22
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2018-05-19
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-05-19
Question
Q: how do I analyze ELISA data? I have performed FOLR2 level in serum.
G. Nelson
Verified customer
Asked: 2017-12-19
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2017-12-19
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2017-09-01
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2017-09-01
