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Product Info Summary
| SKU: | PA1941 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Zinc finger protein GLI2 Gli2 Antibody Picoband®
SKU/Catalog Number
PA1941
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zinc finger protein GLI2 Gli2 Antibody catalog # PA1941. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Zinc finger protein GLI2 Gli2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1941)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Gli2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1941 is reactive to GLI2 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
89 kDa
Calculated molecular weight
167783 MW
Background of GLI-2
GLI2 (Gli-Kruppel Family Member 2), also called ONCOGENE GLI2, is a protein that in humans is encoded by the GLI2 gene. Sequencing of GLI cDNA clones showed the presence of 5 tandem zinc fingers connected by histidine-cysteine links, which indicated that the gene belongs to the family of zinc finger genes related to Kruppel (Kr). The Drosophila gene Kr is a member of the gap class of segmentation genes; thoracic and anterior abdominal segments fail to form in Kr mutant embryos. By fluorescence in situ hybridization, Matsumoto et al. (1996) refined the assignment of the GLI2 gene to chromosome 2q14. Roessler et al. (2005) showed that GLI2-delta-N exhibited potent transcriptional activity in vivo: overexpression in mouse skin led to the formation of hedgehog-independent epithelial downgrowths resembling basal cell carcinomas, which in humans are associated with constitutive hedgehog signaling.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1941 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: Human Placenta Tissue, MCF-7 Cell, HELA Cell, SKOV Cell, HT1080 Cell, A549 Cell,
Validation Images & Assay Conditions
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Anti-Gli2 antibody, PA1941, Western blotting
WB: Human Placenta Tissue Lysate
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Anti-Gli2 antibody, PA1941, Western blotting
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: SKOV Cell Lysate
Lane 4: HT1080 Cell Lysate
Lane 5: A549 Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For GLI2 (Source: Uniprot.org, NCBI)
Gene Name
GLI2
Full Name
Zinc finger protein GLI2
Weight
167783 MW
Superfamily
GLI C2H2-type zinc-finger protein family
Alternative Names
Zinc finger protein GLI2 ;GLI family zinc finger protein 2 ;Tax helper protein ;GLI2 ;THP ; GLI2 CJS, HPE9, PHS2, THP1, THP2 GLI family zinc finger 2 zinc finger protein GLI2|GLI family zinc finger protein 2|GLI-Kruppel family member GLI2|glioma-associated oncogene family zinc finger 2|oncogene GLI2|tax helper protein 1|tax helper protein 2|tax-responsive element-2 holding protein|tax-responsive element-25-bp sequence binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GLI2, check out the GLI2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GLI2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Zinc finger protein GLI2 Gli2 Antibody Picoband® (PA1941)
Hello CJ!
PA1941 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
O-GlcNAcylation of GLI transcription factors in hyperglycemic conditions augments Hedgehog activity
Xiang W, Jiang T, Guo F, Gong C, Yang K, Wu Y, Huang X, Cheng W, Xu K. Oncol Rep. 2014 Oct;32(4):1622-30. Doi: 10.3892/Or.2014.3372. Epub 2014 Jul 31. Hedgehog Pathway Inhibitor-4 Suppresses Malignant Properties Of Chondrosarcoma Cells By Disturbi...
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Zinc finger protein GLI2 Gli2 Antibody Picoband®
Question
Is there a BSA free version of anti-Gli2 antibody PA1941 available?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Gli2 antibody PA1941 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-09
Question
We are currently using anti-Gli2 antibody PA1941 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
The anti-Gli2 antibody (PA1941) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-12
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-Gli2 antibody PA1941. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-27
Question
Would anti-Gli2 antibody PA1941 work on monkey WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-06-19
Answer
Our lab technicians have not validated anti-Gli2 antibody PA1941 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Gli2 antibody PA1941 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey cervix carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-19
Question
Is a blocking peptide available for product anti-Gli2 antibody (PA1941)?
Verified Customer
Verified customer
Asked: 2019-04-05
Answer
We do provide the blocking peptide for product anti-Gli2 antibody (PA1941). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-04-05
