Click image to see more details
Product Info Summary
| SKU: | PB9870 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Filaggrin/FLG Antibody Picoband®
SKU/Catalog Number
PB9870
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Filaggrin/FLG Antibody Picoband® catalog # PB9870. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Filaggrin/FLG Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9870)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Filaggrin recombinant protein (Position: M1-R261).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9870 is reactive to FLG in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
435 kDa
Calculated molecular weight
435170 MW
Background of Filaggrin
In humans, profillagrin is encoded by the FLG gene, which is part of the S100 fused-type protein (SFTP) family within the epidermal differentiation complex on chromosome 1q21. The protein encoded by this gene is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Mutations in this gene are associated with ichthyosis vulgaris.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9870 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: 22RV1 whole cell
IHC: human tonsil tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Filaggrin using anti-Filaggrin antibody (PB9870).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: 22RV1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Filaggrin antigen affinity purified polyclonal antibody (Catalog # PB9870) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Filaggrin at approximately 435 kDa. The expected band size for Filaggrin is at 435 kDa.
Click image to see more details
Figure 2. IHC analysis of Filaggrin using anti-Filaggrin antibody (PB9870).
Filaggrin was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Filaggrin Antibody (PB9870) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For FLG (Source: Uniprot.org, NCBI)
Gene Name
FLG
Full Name
Filaggrin
Weight
435170 MW
Superfamily
S100-fused protein family
Alternative Names
Filaggrin;FLG; FLG ATOD2 filaggrin filaggrin|epidermal filaggrin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FLG, check out the FLG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FLG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Filaggrin/FLG Antibody Picoband® (PB9870)
Hello CJ!
No publications found for PB9870
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Filaggrin/FLG Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Filaggrin/FLG Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
17 Customer Q&As for Anti-Filaggrin/FLG Antibody Picoband®
Question
We need using your anti-Filaggrin/FLG antibody for formation of the cornified envelope studies. Has this antibody been tested with western blotting on 22rv1 whole cell lysates? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
We appreciate your inquiry. This PB9870 anti-Filaggrin/FLG antibody is tested on 22rv1 whole cell lysates. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-03-06
Question
Will anti-Filaggrin/FLG antibody PB9870 work on primate WB with liver?
C. Moore
Verified customer
Asked: 2020-03-03
Answer
Our lab technicians have not validated anti-Filaggrin/FLG antibody PB9870 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Filaggrin/FLG antibody PB9870 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-03
Question
We have seen staining in human tear. Are there any suggestions? Is anti-Filaggrin/FLG antibody supposed to stain tear positively?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
According to literature tear does express FLG. According to Uniprot.org, FLG is expressed in mammalian vulva, foreskin, tear, liver, among other tissues. Regarding which tissues have FLG expression, here are a few articles citing expression in various tissues:
Foreskin, Pubmed ID: 7612609
Liver, Pubmed ID: 24275569
Tear, Pubmed ID: 25946035
Boster Scientific Support
Answered: 2020-02-04
Question
Can you help my question with product PB9870, anti-Filaggrin/FLG antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9870 anti-Filaggrin/FLG antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-10
Question
Is a blocking peptide available for product anti-Filaggrin/FLG antibody (PB9870)?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
We do provide the blocking peptide for product anti-Filaggrin/FLG antibody (PB9870). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-06
Question
Does anti-Filaggrin/FLG antibody PB9870 work for IHC with liver?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
According to the expression profile of liver, FLG is highly expressed in liver. So, it is likely that anti-Filaggrin/FLG antibody PB9870 will work for IHC with liver.
Boster Scientific Support
Answered: 2019-06-26
Question
I was wanting to use your anti-Filaggrin/FLG antibody for IHC for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2019-06-19
Answer
It shows on the product datasheet, PB9870 anti-Filaggrin/FLG antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-19
Question
Do you have a BSA free version of anti-Filaggrin/FLG antibody PB9870 available?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Filaggrin/FLG antibody PB9870 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-11
Question
I would like to test anti-Filaggrin/FLG antibody PB9870 on human liver for research purposes, then I may be interested in using anti-Filaggrin/FLG antibody PB9870 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-04-12
Answer
The products we sell, including anti-Filaggrin/FLG antibody PB9870, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-04-12
Question
Our team were satisfied with the WB result of your anti-Filaggrin/FLG antibody. However we have observed positive staining in mammalian vulva cytoplasmic granule using this antibody. Is that expected? Could you tell me where is FLG supposed to be expressed?
N. Baker
Verified customer
Asked: 2019-03-22
Answer
Based on literature, mammalian vulva does express FLG. Generally FLG expresses in cytoplasmic granule. Regarding which tissues have FLG expression, here are a few articles citing expression in various tissues:
Foreskin, Pubmed ID: 7612609
Liver, Pubmed ID: 24275569
Tear, Pubmed ID: 25946035
Boster Scientific Support
Answered: 2019-03-22
Question
Does PB9870 anti-Filaggrin/FLG antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-02-19
Answer
As indicated on the product datasheet, PB9870 anti-Filaggrin/FLG antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-02-19
Question
Is this PB9870 anti-Filaggrin/FLG antibody reactive to the isotypes of FLG?
Verified Customer
Verified customer
Asked: 2019-01-25
Answer
The immunogen of PB9870 anti-Filaggrin/FLG antibody is E. coli-derived human Filaggrin recombinant protein (Position: M1-R261). . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-01-25
Question
Here is the WB image, lot number and protocol we used for liver using anti-Filaggrin/FLG antibody PB9870. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-12-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-27
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Filaggrin/FLG antibody PB9870. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-05-01
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-01
Question
I see that the anti-Filaggrin/FLG antibody PB9870 works with IHC, what is the protocol used to produce the result images on the product page?
K. Gonzalez
Verified customer
Asked: 2017-10-12
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-10-12
Question
We ordered your anti-Filaggrin/FLG antibody for WB on liver a few years ago. I am using human, and I plan to use the antibody for IHC next. you antibody examining liver as well as tear in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2017-08-08
Answer
I took a look at the website and datasheets of our anti-Filaggrin/FLG antibody and it seems that PB9870 has been validated on human in both WB and IHC. Thus PB9870 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-08-08
Question
We are currently using anti-Filaggrin/FLG antibody PB9870 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?
B. Yang
Verified customer
Asked: 2014-07-28
Answer
The anti-Filaggrin/FLG antibody (PB9870) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-07-28
