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Product Info Summary
| SKU: | M01063 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC |
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Product info
Product Name
Anti-Filaggrin (Keratinocyte Differentiation Marker) FLG Monoclonal Antibody
SKU/Catalog Number
M01063
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-Filaggrin (Keratinocyte Differentiation Marker) FLG Monoclonal Antibody (Catalog # M01063). Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-Filaggrin (Keratinocyte Differentiation Marker) FLG Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01063)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: FLG/1562
Isotype
IgG1, kappa
Immunogen
Recombinant human Filaggrin protein fragment (aa 198-288) (exact sequence is proprietary)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M01063 is reactive to FLG in Human
Reconstitution
Reconstitute with distilled water.
Observed Molecular Weight
68 kDa
Calculated molecular weight
435170 MW
Background of Filaggrin
Filaggrin is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules.Active filaggrin is present at a level of the epidermis where keratinocytes are in transition between the live nucleated granular layer and the anucleate cornified layer, suggesting that filaggrin aids in the terminal differentiation process by facilitating apoptotic machinery.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01063 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Skin stained with Anti-Filaggrin Mouse Monoclonal Antibody (FLG/1562).
Protein Target Info & Infographic
Gene/Protein Information For FLG (Source: Uniprot.org, NCBI)
Gene Name
FLG
Full Name
Filaggrin
Weight
435170 MW
Superfamily
S100-fused protein family
Alternative Names
Filaggrin;FLG; FLG ATOD2 filaggrin filaggrin|epidermal filaggrin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FLG, check out the FLG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FLG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Filaggrin (Keratinocyte Differentiation Marker) FLG Monoclonal Antibody (M01063)
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No publications found for M01063
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-Filaggrin (Keratinocyte Differentiation Marker) FLG Monoclonal Antibody
Question
We have seen staining in human mammalian vulva. Are there any suggestions? Is anti-Filaggrin (Keratinocyte Differentiation Marker) Monoclonal antibody supposed to stain mammalian vulva positively?
Z. Li
Verified customer
Asked: 2019-09-03
Answer
According to literature mammalian vulva does express FLG. According to Uniprot.org, FLG is expressed in mammalian vulva, foreskin, tear, liver, among other tissues. Regarding which tissues have FLG expression, here are a few articles citing expression in various tissues:
Foreskin, Pubmed ID: 7612609
Liver, Pubmed ID: 24275569
Tear, Pubmed ID: 25946035
Boster Scientific Support
Answered: 2019-09-03
Question
My colleagues were satisfied with the WB result of your anti-Filaggrin (Keratinocyte Differentiation Marker) Monoclonal antibody. However we have seen positive staining in foreskin cytoplasmic granule using this antibody. Is that expected? Could you tell me where is FLG supposed to be expressed?
T. Walker
Verified customer
Asked: 2019-08-30
Answer
According to literature, foreskin does express FLG. Generally FLG expresses in cytoplasmic granule. Regarding which tissues have FLG expression, here are a few articles citing expression in various tissues:
Foreskin, Pubmed ID: 7612609
Liver, Pubmed ID: 24275569
Tear, Pubmed ID: 25946035
Boster Scientific Support
Answered: 2019-08-30
Question
We are currently using anti-Filaggrin (Keratinocyte Differentiation Marker) Monoclonal antibody M01063 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?
W. Zhang
Verified customer
Asked: 2014-05-22
Answer
The anti-Filaggrin (Keratinocyte Differentiation Marker) Monoclonal antibody (M01063) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-05-22
