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Product Info Summary
| SKU: | M03178 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Fibrillarin FBL Rabbit Monoclonal Antibody
View all Fibrillarin Antibodies
SKU/Catalog Number
M03178
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Fibrillarin FBL Rabbit Monoclonal Antibody catalog # M03178. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Fibrillarin FBL Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03178)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DIO-6
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Fibrillarin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03178 is reactive to FBL in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
36 kDa
Calculated molecular weight
34,36 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03178 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:100
ICC/IF 1:50-1:100
IP :
1:30
FC 1:50
Positive Control
WB: human Hela whole cell, human 293T whole cell, human MCF-7 whole cell, human K562 whole cell, rat liver tissue, rat spleen tissue, mouse liver tissue, mouse spleen tissue
IHC: human testis tissue
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human testis, using Fibrillarin Antibody.
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Figure 1. Western blot analysis of Fibrillarin using anti-Fibrillarin antibody (M03178).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat spleen tissue lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Fibrillarin antigen affinity purified monoclonal antibody (Catalog # M03178) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Fibrillarin at approximately 36 kDa. The expected band size for Fibrillarin is at 34,36 kDa.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis of Hela cells, using Fibrillarin Antibody .
Protein Target Info & Infographic
Gene/Protein Information For FBL (Source: Uniprot.org, NCBI)
Gene Name
FBL
Full Name
rRNA 2'-O-methyltransferase fibrillarin
Weight
34,36 kDa
Superfamily
methyltransferase superfamily
Alternative Names
rRNA 2'-O-methyltransferase fibrillarin;2.1.1.-;34 kDa nucleolar scleroderma antigen;Histone-glutamine methyltransferase;FBL;FIB1, FLRN; FBL FIB, FLRN, Nop1, RNU3IP1 fibrillarin rRNA 2-O-methyltransferase fibrillarin|34 kDa nucleolar scleroderma |34-kD nucleolar scleroderma |RNA, U3 small nucleolar interacting protein 1|U6 snRNA 2-O-methyltransferase fibrillarin|histone-glutamine methyltransferase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FBL, check out the FBL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FBL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Fibrillarin FBL Rabbit Monoclonal Antibody (M03178)
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