Anti-Fibrillarin FBL Rabbit Monoclonal Antibody

Product Name

Anti-Fibrillarin FBL Rabbit Monoclonal Antibody

View all Fibrillarin Antibodies

SKU/Catalog Number

M03178

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Fibrillarin FBL Rabbit Monoclonal Antibody catalog # M03178. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Fibrillarin FBL Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03178)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

DIO-6

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Fibrillarin

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M03178 is reactive to FBL in Human, Mouse, Rat

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

70 kDa

Calculated molecular weight

33784 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M03178 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:100
ICC/IF 1:50-1:100
IP :
1:30
FC 1:50

Positive Control

Validation Images & Assay Conditions

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Immunohistochemical analysis of paraffin-embedded human testis, using Fibrillarin Antibody.

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Figure 1. Western blot analysis of Fibrillarin using anti-Fibrillarin antibody (M03178).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat spleen tissue lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Fibrillarin antigen affinity purified monoclonal antibody (Catalog # M03178) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Fibrillarin at approximately 36 kDa. The expected band size for Fibrillarin is at 34,36 kDa.

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Immunofluorescent analysis using the Antibody at 1:50 dilution.

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Immunofluorescent analysis of Hela cells, using Fibrillarin Antibody .

Gene/Protein Information For FBL (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.1.1,34-kD nucleolar scleroderma antigen; EC 2.1.1.-; EC 2.1.1.37; FIB; FIB1,34 kDa nucleolar scleroderma antigen; fibrillarin; FLRNrRNA 2'-O-methyltransferase fibrillarin; RNA, U3 small nucleolar interacting protein 1; RNU3IP1 FBL FIB, FLRN, Nop1, RNU3IP1 fibrillarin rRNA 2-O-methyltransferase fibrillarin|34 kDa nucleolar scleroderma antigen|34-kD nucleolar scleroderma antigen|RNA, U3 small nucleolar interacting protein 1|U6 snRNA 2-O-methyltransferase fibrillarin|histone-glutamine methyltransferase

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