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Product Info Summary
| SKU: | M03178-3 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Fibrillarin FBL Monoclonal Antibody
View all Fibrillarin Antibodies
SKU/Catalog Number
M03178-3
Size
100ul
Description
Boster Bio Anti-Fibrillarin FBL Monoclonal Antibody catalog # M03178-3. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Fibrillarin FBL Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03178-3)
Host
Mouse
Contents
Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3
Clonality
Monoclonal
Immunogen
Recombinant full length human sequence expressed in and purified from E. coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03178-3 is reactive to FBL in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
33784 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03178-3 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1,000. IF/ICC and IHC: 1:1,000-1:5,000.
Validation Images & Assay Conditions
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Western blot analysis of different tissue and cell line lysates using mouse mAb to fibrillarin M03178-3, dilution 1:2,000, in green: [1] protein standard (red), [2] rat whole brain, [3] mouse whole brain, [4] NIH-3T3 cells,[5] HEK293, [6] HeLa, and [7] SH-SY5Y cells. Strong band at ~35kDa corresponds
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Immunofluorescent analysis of (A) C6 rat glioma cells and (B) HEK293 human embryonic kidney cells stained with mouse mAb to fibrillarin, M03178-3, dilution 1:1,000 in red, in both cases costained with chicken pAb to vimentin, dilution 1:10,000 in green. The blue is DAPI staining of nuclear DNA. The M03178-3 antibody detects fibrillarin protein localized in nucleoli while the other antibody produces strong staining of cytoplasmic intermediate filaments.
Protein Target Info & Infographic
Gene/Protein Information For FBL (Source: Uniprot.org, NCBI)
Gene Name
FBL
Full Name
rRNA 2'-O-methyltransferase fibrillarin
Weight
33784 MW
Superfamily
methyltransferase superfamily
Alternative Names
rRNA 2'-O-methyltransferase fibrillarin;2.1.1.-;34 kDa nucleolar scleroderma antigen;Histone-glutamine methyltransferase;FBL;FIB1, FLRN; FBL FIB, FLRN, Nop1, RNU3IP1 fibrillarin rRNA 2-O-methyltransferase fibrillarin|34 kDa nucleolar scleroderma |34-kD nucleolar scleroderma |RNA, U3 small nucleolar interacting protein 1|U6 snRNA 2-O-methyltransferase fibrillarin|histone-glutamine methyltransferase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FBL, check out the FBL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FBL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Fibrillarin FBL Monoclonal Antibody (M03178-3)
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