Anti-FEN1 Antibody Picoband®

FEN-1 antibody

Boster Bio Anti-FEN1 Antibody Picoband® catalog # A01484-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: A01484-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-FEN1 Antibody Picoband®

View all FEN-1 Antibodies

SKU/Catalog Number

A01484-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-FEN1 Antibody Picoband® catalog # A01484-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-FEN1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01484-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human FEN1 recombinant protein (Position: Q4-E300).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01484-1 is reactive to FEN1 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

43 kDa

Calculated molecular weight

75679 MW

Background of FEN-1

Flap endonuclease 1 is an enzyme that in humans is encoded by the FEN1 gene. It is mapped to 11q12.2. The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A01484-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -

Positive Control

WB: human Hela whole cell, human Daudi whole cell, human U20S whole cell, human K562 whole cell, rat PC-12 whole cell, mouse SP2/0 whole cell
IHC: human liver cancer tissue, human ovarian serous adenocarcinoma tissue, human esophageal squamous carcinoma tissue, human lung adenocarcinoma tissue, human rectal cancer tissue
ICC/IF: U20S cell
IF: human intestinal cancer tissue
FCM: K562 cell

Validation Images & Assay Conditions

Gene/Protein Information For FEN1 (Source: Uniprot.org, NCBI)

Gene Name

FEN1

Full Name

Flap endonuclease 1

Weight

75679 MW

Superfamily

XPG/RAD2 endonuclease family

Alternative Names

Flap endonuclease 1; FEN-1; DNase IV; Flap structure-specific endonuclease 1; Maturation factor 1; MF1; hFEN-1 FEN1 FEN-1, MF1, RAD2 flap structure-specific endonuclease 1 flap endonuclease 1|DNase IV|maturation factor-1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on FEN1, check out the FEN1 Infographic

FEN1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for FEN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for A01484-1

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-FEN1 Antibody Picoband®?

Submit a review and receive an Amazon gift card.

  • $30 for a review with an image

0 Reviews For Anti-FEN1 Antibody Picoband®

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

1 Customer Q&As for Anti-FEN1 Antibody Picoband®

Question

We are currently using anti-FEN1 antibody A01484-1 for rat tissue, and we are well pleased with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2019-04-04

Answer

The anti-FEN1 antibody (A01484-1) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-04-04

Order DetailsPrice
A01484-1

100μg

$370
A01484-1-10ug

10μg sample (liquid)

$99
A01484-1-Biotin

100 μg Biotin conjugated

$570
A01484-1-Cy3

100 μg Cy3 conjugated

$570
A01484-1-Dylight488

100 μg Dylight488 conjugated

$570
A01484-1-Dylight550

100 μg Dylight550 conjugated

$570
A01484-1-Dylight594

100 μg Dylight594 conjugated

$570
A01484-1-FITC

100 μg FITC conjugated

$570
A01484-1-HRP

100 μg HRP conjugated

$570
A01484-1-APC

100 μg APC conjugated

$670
A01484-1-PE

100 μg PE conjugated

$670
A01484-1-iFluor647

100 μg iFluor647 conjugated

$670
A01484-1-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
In stock
Order Product
A01484-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.