Anti-FLT3(Ab-591) Antibody

Product Name

Anti-FLT3(Ab-591) Antibody

View all Flt-3/Flk-2/CD135 Antibodies

SKU/Catalog Number

A00188

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-FLT3(Ab-591) Antibody (Catalog # A00188). Tested in WB, IF applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-FLT3(Ab-591) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00188)

Host

Rabbit

Contents

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Peptide sequence around aa.589~593 (Y-F-Y-V-D) derived from Human FLT3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00188 is reactive to FLT3 in Human

Reconstitution

Calculated molecular weight

112903 MW

Background of Flt-3/Flk-2/CD135

FLT3 encodes a class III receptor tyrosine kinase that regulates hematopoiesis. The receptor consists of an extracellular domain composed of five immunoglobulin-like domains, one transmembrane region, and a cytoplasmic kinase domain split into two parts by a kinase-insert domain. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.

Sekine S et,al. (2008) J Immunol. ;180(12):8126-34
Pratz K et,al. (2008) Leuk Lymphoma. 2008 ;49(5):852-63.
Al Shaer L et,al. (2008) Br J Haematol. 141(4):483-93.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00188 is guaranteed for IF, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Predicted MW: 130 160 kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Validation Images & Assay Conditions

Gene/Protein Information For FLT3 (Source: Uniprot.org, NCBI)

Alternative Names

Receptor-type tyrosine-protein kinase FLT3;2.7.10.1;FL cytokine receptor;Fetal liver kinase-2;FLK-2;Fms-like tyrosine kinase 3;FLT-3;Stem cell tyrosine kinase 1;STK-1;CD135;FLT3;CD135, FLK2, STK1; FLT3 CD135, FLK-2, FLK2, STK1 fms related receptor tyrosine kinase 3 receptor-type tyrosine-protein kinase FLT3|CD135 |FL cytokine receptor|fetal liver kinase 2|fms related tyrosine kinase 3|fms-like tyrosine kinase 3|growth factor receptor tyrosine kinase type III|stem cell tyrosine kinase 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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