Anti-Flap endonuclease 1 FEN1 Antibody

Product Name

Anti-Flap endonuclease 1 FEN1 Antibody

View all FEN-1 Antibodies

SKU/Catalog Number

A01484

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Flap endonuclease 1 FEN1 Antibody (Catalog # A01484). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Flap endonuclease 1 FEN1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01484)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human FEN1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01484 is reactive to FEN1 in Human, Mouse, Rat

Reconstitution

Calculated molecular weight

42593 MW

Background of FEN-1

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.

Murray J.M., Mol. Cell. Biol. 14:4878-4888(1994).
Hiraoka L.R., Genomics 25:220-225(1995).
Taylor T.D., Nature 440:497-500(2006).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01484 is guaranteed for IF, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500

Validation Images & Assay Conditions

Gene/Protein Information For FEN1 (Source: Uniprot.org, NCBI)

Alternative Names

Flap endonuclease 1 ;FEN-1 ;3.1.-.- ;DNase IV;Flap structure-specific endonuclease 1 ;Maturation factor 1;MF1;hFEN-1;FEN1 ;RAD2; FEN1 FEN-1, MF1, RAD2 flap structure-specific endonuclease 1 flap endonuclease 1|DNase IV|maturation factor-1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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