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Product Info Summary
| SKU: | M00122-3 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PARP PARP1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00122-3
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PARP PARP1 Rabbit Monoclonal Antibody catalog # M00122-3. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PARP PARP1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00122-3)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CFD-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PARP
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00122-3 is reactive to PARP1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
113 kDa
Calculated molecular weight
113 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00122-3 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Positive Control
WB: human Hela whole cell, human HepG2 whole cell, human SIHA whole cell, human K562 whole cell, human 293T whole cell, human SH-SY5Y whole cell
ICC/IF: Hela cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PARP1 using anti-PARP1 antibody (M00122-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human SIHA whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: human 293T whole cell lysates,
Lane 6: human SH-SY5Y whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PARP1 antigen affinity purified monoclonal antibody (M00122-3) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PARP1 at approximately 113 kDa. The expected band size for PARP1 is at 113 kDa.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human testis, using PARP Antibody.
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Immunofluorescent analysis of Hela cells, using PARP Antibody .
Protein Target Info & Infographic
Gene/Protein Information For PARP1 (Source: Uniprot.org, NCBI)
Gene Name
PARP1
Full Name
Poly [ADP-ribose] polymerase 1
Weight
113 kDa
Alternative Names
Poly [ADP-ribose] polymerase 1;PARP-1;2.4.2.30;ADP-ribosyltransferase diphtheria toxin-like 1;ARTD1;NAD (+) ADP-ribosyltransferase 1;ADPRT 1;Poly[ADP-ribose] synthase 1;PARP1;ADPRT, PPOL; PARP1 ADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PPOL, pADPRT-1 poly(ADP-ribose) polymerase 1 poly [ADP-ribose] polymerase 1|ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)|ADP-ribosyltransferase NAD(+)|ADP-ribosyltransferase diphtheria toxin-like 1|DNA ADP-ribosyltransferase PARP1|NAD(+) ADP-ribosyltransferase 1|poly (ADP-ribose) polymerase family, member 1|poly(ADP-ribose) synthetase|poly(ADP-ribosyl)transferase|poly[ADP-ribose] synthase 1|protein poly-ADP-ribosyltransferase PARP1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PARP1, check out the PARP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PARP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PARP PARP1 Rabbit Monoclonal Antibody (M00122-3)
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-PARP PARP1 Rabbit Monoclonal Antibody
Question
We are currently using anti-PARP Rabbit Monoclonal antibody M00122-3 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
The anti-PARP Rabbit Monoclonal antibody (M00122-3) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-18
Question
Is a blocking peptide available for product anti-PARP Rabbit Monoclonal antibody (M00122-3)?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
We do provide the blocking peptide for product anti-PARP Rabbit Monoclonal antibody (M00122-3). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-13
Question
Is there a BSA free version of anti-PARP Rabbit Monoclonal antibody M00122-3 available?
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-PARP Rabbit Monoclonal antibody M00122-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-07
Question
I would like to test anti-PARP Rabbit Monoclonal antibody M00122-3 on mouse liver for research purposes, then I may be interested in using anti-PARP Rabbit Monoclonal antibody M00122-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
The products we sell, including anti-PARP Rabbit Monoclonal antibody M00122-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-09
Question
Would anti-PARP Rabbit Monoclonal antibody M00122-3 work on primate WB with brain?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
Our lab technicians have not tested anti-PARP Rabbit Monoclonal antibody M00122-3 on primate. You can run a BLAST between primate and the immunogen sequence of anti-PARP Rabbit Monoclonal antibody M00122-3 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-17
Question
Our lab were content with the WB result of your anti-PARP Rabbit Monoclonal antibody. However we have seen positive staining in cervix carcinoma nucleus using this antibody. Is that expected? Could you tell me where is PARP1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
Based on literature, cervix carcinoma does express PARP1. Generally PARP1 expresses in nucleus. Regarding which tissues have PARP1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, and Ovarian carcinoma, Pubmed ID: 17177976
Embryonic kidney, Pubmed ID: 17525332
Fibroblast, Pubmed ID: 3120710, 2824474, 2891139
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2019-09-10
Question
Please see the WB image, lot number and protocol we used for liver using anti-PARP Rabbit Monoclonal antibody M00122-3. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-22
Question
My question regarding product M00122-3, anti-PARP Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00122-3 anti-PARP Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-16
Question
We have been able to see staining in human colon carcinoma ovarian carcinoma. Any tips? Is anti-PARP Rabbit Monoclonal antibody supposed to stain colon carcinoma ovarian carcinoma positively?
Verified Customer
Verified customer
Asked: 2019-04-25
Answer
From what I have seen in literature colon carcinoma ovarian carcinoma does express PARP1. From what I have seen in Uniprot.org, PARP1 is expressed in heart left ventricle, fibroblast, brain, colon carcinoma ovarian carcinoma, embryonic kidney, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have PARP1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, and Ovarian carcinoma, Pubmed ID: 17177976
Embryonic kidney, Pubmed ID: 17525332
Fibroblast, Pubmed ID: 3120710, 2824474, 2891139
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2019-04-25
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-PARP Rabbit Monoclonal antibody M00122-3. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-01-08
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-01-08
Question
I am interested in using your anti-PARP Rabbit Monoclonal antibody for positive regulation of cardiac muscle hypertrophy studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-11-29
Answer
I appreciate your inquiry. This M00122-3 anti-PARP Rabbit Monoclonal antibody is validated on jurkat cell lysate. It is guaranteed to work for IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-11-29
Question
Would anti-PARP Rabbit Monoclonal antibody M00122-3 work for WB with liver?
Verified Customer
Verified customer
Asked: 2018-09-03
Answer
According to the expression profile of liver, PARP1 is highly expressed in liver. So, it is likely that anti-PARP Rabbit Monoclonal antibody M00122-3 will work for WB with liver.
Boster Scientific Support
Answered: 2018-09-03
Question
Is this M00122-3 anti-PARP Rabbit Monoclonal antibody reactive to the isotypes of PARP1?
Verified Customer
Verified customer
Asked: 2018-07-24
Answer
The immunogen of M00122-3 anti-PARP Rabbit Monoclonal antibody is A synthesized peptide derived from human PARP. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-07-24
Question
Our lab used your anti-PARP Rabbit Monoclonal antibody for WB on liver in the past. I am using mouse, and I plan to use the antibody for IF next. you antibody examining liver as well as cervix carcinoma in our next experiment. Could give a recommendation on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2017-09-06
Answer
I took a look at the website and datasheets of our anti-PARP Rabbit Monoclonal antibody and it appears that M00122-3 has been validated on mouse in both WB and IF. Thus M00122-3 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-09-06
Question
Would M00122-3 anti-PARP Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
L. Singh
Verified customer
Asked: 2017-01-23
Answer
It shows on the product datasheet, M00122-3 anti-PARP Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-01-23
Question
I see that the anti-PARP Rabbit Monoclonal antibody M00122-3 works with WB, what is the protocol used to produce the result images on the product page?
W. Johnson
Verified customer
Asked: 2016-09-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-09-23
Question
I was wanting to use your anti-PARP Rabbit Monoclonal antibody for WB for mouse liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse liver identification?
N. Jha
Verified customer
Asked: 2014-08-19
Answer
As indicated on the product datasheet, M00122-3 anti-PARP Rabbit Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-08-19
