Anti-F-box only protein 4 FBXO4 Antibody

Product Name

Anti-F-box only protein 4 FBXO4 Antibody

View all FBXO4 Antibodies

SKU/Catalog Number

A08044

Size

100uL

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-F-box only protein 4 FBXO4 Antibody (Catalog # A08044). Tested in IF, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-F-box only protein 4 FBXO4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08044)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

Antiserum

Immunogen

This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide derived from sequences unique to the N-terminus of FBX4 and conserved between the human and murine proteins (see link below for the full-length sequence of the mouse gene product).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A08044 is reactive to FBXO4 in Human, Mouse

Applications

A08044 is guaranteed for IF, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

191100 MW

Background of FBXO4

Skp/Cullin/F-box (SCF) complexes are part of a large family of E3 ubiquitin ligases in which F-box proteins determine substrate specificity. Substrate selection by F-box proteins may also be aided by accessory cofactors. The F-box only protein 4, FBX4, interacts via its F-box with SKP1 and CUL1 to form SCFFBX4 ubiquitin ligase. The activity of the SCFFBX4 complex can be regulated by aB crystallin, a small heat shock protein which serves as a specificity cofactor for FBX4 dependent ubiquitination. FBX4 together with aB crystallin (SCFFBX4-aB crystallin) promotes the ubiquitination of cyclin D1 phosphorylated at Thr-286. In addition, the SCFFBX4-aB crystallin ubiquitin ligase has been implicated in the ubiquitination of unknown substrates in desmin-related myopathy. SCFFBX4, in the absence of aB crystallin, also ubiquitinates the telomeric protein PIN2/TRF1.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:5,000 - 1:25,000
WB: 1:500 – 1:3000

Validation Images & Assay Conditions

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Whole cell extracts prepared from U2OS cells, or insect Sf9 cells expressing Flag-FBX4, were resolved by SDS-PAGE and transferred to nitrocellulose.FBX4

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Immunocytochemistry staining of NIH-3T3 cells

Gene/Protein Information For FBXO4 (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp547N213; F-box protein 4; F-box protein Fbx4; FBX4F-box only protein 4; FLJ10141 FBXO4 FBX4 F-box protein 4 F-box only protein 4|F-box protein Fbx4

For more info on FBXO4, check out the FBXO4 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for FBXO4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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