Anti-NOTCH4 Antibody Picoband®

Product Name

Anti-NOTCH4 Antibody Picoband®

View all Notch-4 Antibodies

SKU/Catalog Number

PA1831

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-NOTCH4 Antibody catalog # PA1831. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NOTCH4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1831)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human NOTCH4.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1831 is reactive to NOTCH4 in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

210 kDa

Calculated molecular weight

209622 MW

Background of Notch-4

NOTCH4 (NOTCH, DROSOPHILA, HOMOLOG OF, 4) also known as INT3 or NOTCH3, FORMERLY, is a member of the Notch family. In situ hybridization revealed that Notch4 transcripts are primarily restricted to endothelial cells in embryonic and adult life, suggesting a specific role forNotch4 during development of vertebrate endothelium. The sequences of the mouse and human NOTCH4 proteins are 82% identical. Northern blot analysis revealed that NOTCH4 (S) is the major transcript and is expressed in a wide variety of tissues. Fluorescence in situ hybridization confirmed the location of the NOTCH4 gene at chromosome 6p21.3. In linkage disequilibrium mapping of the MHC region in 80 British parent-offspring trios, Wei and Hemmings (2000) found that NOTCH4 was highly associated with schizophrenia. Repression of Notch4 resolved ataxia and reversed the disease progression, demonstrating that Notch4 is not only sufficient to induce but also required to sustain the disease. 

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1831 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Positive Control

WB: A549 Whole Cell, SMMC Whole Cell, HELA Whole Cell

Validation Images & Assay Conditions

Gene/Protein Information For NOTCH4 (Source: Uniprot.org, NCBI)

Alternative Names

Neurogenic locus notch homolog protein 4;Notch 4;hNotch4;Notch 4 extracellular truncation;Notch 4 intracellular domain;NOTCH4;INT3; NOTCH4 INT3 notch receptor 4 neurogenic locus notch homolog protein 4|Notch homolog 4|notch 4

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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