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Product Info Summary
| SKU: | A08518 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Fat Free VPS51 Antibody
SKU/Catalog Number
A08518
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Fat Free VPS51 Antibody (Catalog # A08518). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Fat Free antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Fat Free VPS51 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08518)
Host
Rabbit
Contents
Fat Free Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Fat Free antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human Fat Free. The immunogen is located within amino acids 620 - 670 of Fat Free.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least two alternatively spliced isoforms are known to exist; this antibody will detect both isoforms.
Reactive Species
A08518 is reactive to VPS51 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
86042 MW
Background of VPS51
Fat Free, also known as ANG2, was identified though a yeast two-hybrid screen using VP53. It is thought to be a structural and functional homolog of the yeast protein VPS51, a protein that together with VPS52, VPS53, and VPS54 form the Golgi-associated retrograde protein (GARP) complex that mediates the tethering and fusion of endosome-derived transport carriers to the trans-Golgi network (TGN). Similar to depletion of any of the VPS52, VPS53, or VPS54, depletion of Fat Free using RNAi impairs protein retrieval to the TGN indicating that Fat Free is missing component of the GARP complex in most eukaryotes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08518 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Fat Free antibody can be used for detection of Fat Free by Western blot at 1 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Positive Control
Validation Images & Assay Conditions
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Western blot analysis of Fat Free in mouse brain tissue lysate with Fat Free antibody at 1 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For VPS51 (Source: Uniprot.org, NCBI)
Gene Name
VPS51
Full Name
Vacuolar protein sorting-associated protein 51 homolog
Weight
86042 MW
Superfamily
VPS51 family
Alternative Names
ANG2; ANG3; C11orf2; C11orf3; chromosome 11 open reading frame 2; FFR; protein fat-free homolog; vacuolar protein sorting 51 homolog (S. cerevisiae) VPS51 ANG2, ANG3, C11orf2, C11orf3, FFR, PCH13 VPS51 subunit of GARP complex vacuolar protein sorting-associated protein 51 homolog|VPS51, GARP complex subunit|another new gene 2 protein|protein fat-free homolog|vacuolar protein sorting 51 homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VPS51, check out the VPS51 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VPS51: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Fat Free VPS51 Antibody (A08518)
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