Anti-FAM59A GAREM1 Antibody

Product Name

Anti-FAM59A GAREM1 Antibody

View all FAM59A Antibodies

SKU/Catalog Number

A12630

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-FAM59A GAREM1 Antibody (Catalog # A12630). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

FAM59A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-FAM59A GAREM1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12630)

Host

Rabbit

Contents

FAM59A Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

FAM59A antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human FAM59A. The immunogen is located within amino acids 630 - 680 of FAM59A.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

AP3M1 antibody may cross-react with AP3M2.

Reactive Species

A12630 is reactive to GAREM1 in Human, Mouse, Rat

Applications

A12630 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

97186 MW

Background of FAM59A

FAM59A, also known as GAREM, was initially identified through a mass spectroscoptic method that measures different phosphotyrosine states of proteins in response to different cell stimuli. FAM59A is a downstream molecule in the EGF signaling pathway that is tyrosine-phosphorylated, and this phosphorylation is needed for the binding of Grb2, an adaptor protein crucial to the transduction of growth signals from the plasma membrane to the nucleus. ERK activation in response to EGF stimulation is regulated by FAM59A in COS-7 and HeLa cells, and the overexpression of FAM59A stimulated cell proliferation and colony formation in soft agar, suggesting that FAM59A might be a critical protein with roles in ligand-mediated signaling pathway of the EGF receptor and the tumorigenesis of cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

FAM59A antibody can be used for detection of FAM59A by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Click image to see more details

Western blot analysis of FAM59A in rat liver tissue lysate with FAM59A antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.

Click image to see more details

Immunohistochemistry of PLEKHM3 in human brain tissue with PLEKHM3 antibody at 5 μg/mL.

Click image to see more details

Immunofluorescence of FAM59A in Human Liver cells with FAM59A antibody at 20 μg/mL.

Gene/Protein Information For GAREM1 (Source: Uniprot.org, NCBI)

Alternative Names

Chromosome 18 Open Reading Frame 11; chromosome 18 open reading frame 11, family with sequence; FAM59A; Family With Sequence Similarity 59, Member A; FLJ21610; GAREM; Gm944; GRB2 associated, regulator of MAPK1; Grb2-Associated And Regulator Of Erk/MAPK; GRB2-Associated And Regulator Of MAPK Protein; GRB2-Associated And Regulator Of MAPK1; Protein FAM59A GAREM1 C18orf11, FAM59A, GAREM, Gm944 GRB2 associated regulator of MAPK1 subtype 1 GRB2-associated and regulator of MAPK protein 1|GRB2 associated regulator of MAPK1 1|GRB2 associated, regulator of MAPK1|GRB2-associated and regulator of MAPK1|Grb2-associated and regulator of Erk/MAPK|family with sequence similarity 59, member A|protein FAM59A

For more info on GAREM1, check out the GAREM1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GAREM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the GAREM1 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis