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Product Info Summary
| SKU: | A12630 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-FAM59A GAREM1 Antibody
SKU/Catalog Number
A12630
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-FAM59A GAREM1 Antibody (Catalog # A12630). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
FAM59A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-FAM59A GAREM1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12630)
Host
Rabbit
Contents
FAM59A Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
FAM59A antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human FAM59A. The immunogen is located within amino acids 630 - 680 of FAM59A.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
AP3M1 antibody may cross-react with AP3M2.
Reactive Species
A12630 is reactive to FAM59A in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
97186 MW
Background of FAM59A
FAM59A, also known as GAREM, was initially identified through a mass spectroscoptic method that measures different phosphotyrosine states of proteins in response to different cell stimuli. FAM59A is a downstream molecule in the EGF signaling pathway that is tyrosine-phosphorylated, and this phosphorylation is needed for the binding of Grb2, an adaptor protein crucial to the transduction of growth signals from the plasma membrane to the nucleus. ERK activation in response to EGF stimulation is regulated by FAM59A in COS-7 and HeLa cells, and the overexpression of FAM59A stimulated cell proliferation and colony formation in soft agar, suggesting that FAM59A might be a critical protein with roles in ligand-mediated signaling pathway of the EGF receptor and the tumorigenesis of cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12630 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
FAM59A antibody can be used for detection of FAM59A by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of FAM59A in rat liver tissue lysate with FAM59A antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of PLEKHM3 in human brain tissue with PLEKHM3 antibody at 5 μg/mL.
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Immunofluorescence of FAM59A in Human Liver cells with FAM59A antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For FAM59A (Source: Uniprot.org, NCBI)
Gene Name
FAM59A
Full Name
GRB2-associated and regulator of MAPK protein 1
Weight
97186 MW
Superfamily
GAREM family
Alternative Names
Gm944, FAM59A, C18orf11, GRB2-associated and regulator of MAPK protein, GRB2-associated and regulator of MAPK1 GAREM1 C18orf11, FAM59A, GAREM, Gm944 GRB2 associated regulator of MAPK1 subtype 1 GRB2-associated and regulator of MAPK protein 1|GRB2 associated regulator of MAPK1 1|GRB2 associated, regulator of MAPK1|GRB2-associated and regulator of MAPK1|Grb2-associated and regulator of Erk/MAPK|family with sequence similarity 59, member A|protein FAM59A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FAM59A, check out the FAM59A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FAM59A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FAM59A GAREM1 Antibody (A12630)
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