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Product Info Summary
| SKU: | PB9102 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Csk Antibody Picoband®
SKU/Catalog Number
PB9102
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Csk Antibody Picoband® catalog # PB9102. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Csk Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9102)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CSK recombinant protein (Position: S2-G204). Human CSK shares 99% amino acid (aa) sequence identity with both mouse and rat CSK.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9102 is reactive to CSK in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
51 kDa
Calculated molecular weight
50704 MW
Background of CSK
CSK also known as C-Src kinase, is a non-receptor protein tyrosine kinase protein that in humans is encoded by the CSK gene. It is mapped to 15q24.1. CSK suppresses activity of the Src family of protein kinases by phosphorylation of Src family members at a conserved C-terminal site in Src. Csk's control of the Src family activity is widely thought to be central to regulation of the immune response. CSK can downregulate tyrosine kinase activity of the SRC oncoprotein through tyrosine phosphorylation of the SRC carboxy terminus. Since cell transformation by SRC oncoproteins is caused by various mechanisms that interfere with this phosphorylation, the CSK gene might function as an antioncogene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9102 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: rat testis tissue, rat thymus tissue, mouse liver tissue, human Hela whole cell, human Jurkat whole cell, human A549 whole cell, human MCF-7 whole cell, mouse NIH/3T3 whole cell, mouse NEURO whole cell
IHC: human tonsil tissue, human tonsil tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CSK using anti-CSK antibody (PB9102).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human CSK protein 0.5 ng
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSK antigen affinity purified polyclonal antibody (Catalog # PB9102) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSK at approximately 47 kDa. The expected band size for CSK is at 47 kDa.
Click image to see more details
Figure 2. Western blot analysis of CSK using anti-CSK antibody (PB9102).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates,
Lane 2: rat thymus tissue lysates,
Lane 3: mouse liver tissue lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: human Jurkat whole cell lysates,
Lane 6: human A549 whole cell lysates,
Lane 7: human MCF-7 whole cell lysates,
Lane 8: mouse NIH/3T3 whole cell lysates,
Lane 9: mouse NEURO whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSK antigen affinity purified polyclonal antibody (Catalog # PB9102) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSK at approximately 51 kDa. The expected band size for CSK is at 51 kDa.
Click image to see more details
Figure 3. IHC analysis of CSK using anti-CSK antibody (PB9102). CSK was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CSK Antibody (PB9102) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of Csk using anti-Csk antibody (PB9102).
Csk was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Csk Antibody (PB9102) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For CSK (Source: Uniprot.org, NCBI)
Gene Name
CSK
Full Name
Tyrosine-protein kinase CSK
Weight
50704 MW
Superfamily
protein kinase superfamily
Alternative Names
Tyrosine-protein kinase CSK;2.7.10.2;C-Src kinase;Protein-tyrosine kinase CYL;CSK; CSK C-terminal Src kinase tyrosine-protein kinase CSK|C-Src kinase|CSK, non-receptor tyrosine kinase|c-src tyrosine kinase|protein-tyrosine kinase CYL
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CSK, check out the CSK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CSK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Csk Antibody Picoband® (PB9102)
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Customer Q&As
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6 Customer Q&As for Anti-Csk Antibody Picoband®
Question
Does anti-Csk antibody PB9102 work for WB with brain uterus?
Verified Customer
Verified customer
Asked: 2018-08-01
Answer
According to the expression profile of brain uterus, CSK is highly expressed in brain uterus. So, it is likely that anti-Csk antibody PB9102 will work for WB with brain uterus.
Boster Scientific Support
Answered: 2018-08-01
Question
Will PB9102 anti-Csk antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-03-27
Answer
It shows on the product datasheet, PB9102 anti-Csk antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-03-27
Question
I see that the anti-Csk antibody PB9102 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-03-01
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-03-01
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain uterus using anti-Csk antibody PB9102. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-01-24
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-24
Question
See below the WB image, lot number and protocol we used for brain uterus using anti-Csk antibody PB9102. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-09-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-13
Question
We are currently using anti-Csk antibody PB9102 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2017-08-28
Answer
The anti-Csk antibody (PB9102) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-28
