Click image to see more details
Product Info Summary
| SKU: | PB9674 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-FAK/PTK2 Antibody Picoband®
SKU/Catalog Number
PB9674
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FAK/PTK2 Antibody Picoband® catalog # PB9674. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FAK/PTK2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9674)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human PTK2, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9674 is reactive to PTK2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
125 kDa
Calculated molecular weight
119233 MW
Background of FAK
PTK2 protein tyrosine kinase 2 (PTK2), also known as Focal Adhesion Kinase (FAK), is a protein that, in humans, is encoded by the PTK2 gene. This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene, but the full-length natures of only three of them have been determined.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9674 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human Hela whole cell, human PC-3 whole cellhuman K562 whole cell, human A431 whole cell, human A549 whole cell, human HepG2 whole cell, rat brain tissue, rat C6 whole cell, mouse lung tissue, mouse brain tissue, mouse RAW2647 whole cell, mouse NIH/3T3 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of FAK/PTK2 using anti-FAK/PTK2 antibody (PB9674).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human PC-3 whole cell lysates.
Lane 3: human K562 whole cell lysates,
Lane 4: human A431 whole cell lysates,
Lane 5: human A549 whole cell lysates,
Lane 6: human HepG2 whole cell lysates,
Lane 7: rat brain tissue lysates,
Lane 8: rat C6 whole cell lysates,
Lane 9: mouse lung tissue lysates,
Lane 10: mouse brain tissue lysates,
Lane 11: mouse RAW264.7 whole cell lysates,
Lane 12: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAK/PTK2 antigen affinity purified polyclonal antibody (Catalog # PB9674) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FAK/PTK2 at approximately 125 kDa. The expected band size for FAK/PTK2 is at 119 kDa.
Protein Target Info & Infographic
Gene/Protein Information For PTK2 (Source: Uniprot.org, NCBI)
Gene Name
PTK2
Full Name
Focal adhesion kinase 1
Weight
119233 MW
Superfamily
protein kinase superfamily
Alternative Names
Focal adhesion kinase 1;FADK 1;2.7.10.2;Focal adhesion kinase-related nonkinase;FRNK;Protein phosphatase 1 regulatory subunit 71;PPP1R71;Protein-tyrosine kinase 2;p125FAK;pp125FAK;PTK2;FAK, FAK1; PTK2 FADK, FAK, FAK1, FRNK, PPP1R71, p125FAK, pp125FAK protein tyrosine kinase 2 focal adhesion kinase 1|FADK 1|FAK-related non-kinase polypeptide|PTK2 protein tyrosine kinase 2|focal adhesion kinase-related nonkinase|protein phosphatase 1 regulatory subunit 71
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PTK2, check out the PTK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PTK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FAK/PTK2 Antibody Picoband® (PB9674)
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Customer Q&As
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16 Customer Q&As for Anti-FAK/PTK2 Antibody Picoband®
Question
I was wanting to use your anti-FAK/PTK2 antibody for WB for mouse t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse t-cell identification?
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
You can see on the product datasheet, PB9674 anti-FAK/PTK2 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-06
Question
Does anti-FAK/PTK2 antibody PB9674 work on primate WB with brain?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
Our lab technicians have not validated anti-FAK/PTK2 antibody PB9674 on primate. You can run a BLAST between primate and the immunogen sequence of anti-FAK/PTK2 antibody PB9674 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-17
Question
Would anti-FAK/PTK2 antibody PB9674 work for WB with t-cell?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
According to the expression profile of t-cell, PTK2 is highly expressed in t-cell. So, it is likely that anti-FAK/PTK2 antibody PB9674 will work for WB with t-cell.
Boster Scientific Support
Answered: 2019-11-29
Question
I have attached the WB image, lot number and protocol we used for t-cell using anti-FAK/PTK2 antibody PB9674. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-12
Question
Do you have a BSA free version of anti-FAK/PTK2 antibody PB9674 available?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-FAK/PTK2 antibody PB9674 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-25
Question
We are interested in using your anti-FAK/PTK2 antibody for negative regulation of anoikis studies. Has this antibody been tested with western blotting on brain tissue? We would like to see some validation images before ordering.
S. Johnson
Verified customer
Asked: 2019-10-23
Answer
Thank you for your inquiry. This PB9674 anti-FAK/PTK2 antibody is tested on rat lung tissue, tissue lysate, mouse lung tissue, brain tissue, u20s whole cell lysate, hela whole cell lysate, nih3t3 whole cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-10-23
Question
My team were content with the WB result of your anti-FAK/PTK2 antibody. However we have seen positive staining in corpus callosum cell junction using this antibody. Is that expected? Could you tell me where is PTK2 supposed to be expressed?
K. Dhar
Verified customer
Asked: 2019-10-04
Answer
From literature, corpus callosum does express PTK2. Generally PTK2 expresses in cell junction, focal adhesion. cell. Regarding which tissues have PTK2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 8422239, 23503467
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Melanocyte, Pubmed ID: 8247543, 9422762
Placenta, Pubmed ID: 15489334
T-cell, Pubmed ID: 7692878
Trachea, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-10-04
Question
you antibody to test anti-FAK/PTK2 antibody PB9674 on mouse t-cell for research purposes, then I may be interested in using anti-FAK/PTK2 antibody PB9674 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-02
Answer
The products we sell, including anti-FAK/PTK2 antibody PB9674, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-02
Question
Would PB9674 anti-FAK/PTK2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-01
Answer
You can see on the product datasheet, PB9674 anti-FAK/PTK2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-01
Question
We are currently using anti-FAK/PTK2 antibody PB9674 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
The anti-FAK/PTK2 antibody (PB9674) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-18
Question
I see that the anti-FAK/PTK2 antibody PB9674 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-17
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for t-cell using anti-FAK/PTK2 antibody PB9674. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-14
Question
My question regarding product PB9674, anti-FAK/PTK2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
P. Huang
Verified customer
Asked: 2019-02-25
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9674 anti-FAK/PTK2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-02-25
Question
We have seen staining in rat t-cell. Are there any suggestions? Is anti-FAK/PTK2 antibody supposed to stain t-cell positively?
Verified Customer
Verified customer
Asked: 2019-02-06
Answer
From what I have seen in literature t-cell does express PTK2. From what I have seen in Uniprot.org, PTK2 is expressed in corpus callosum, t-cell, brain, trachea, placenta, osteosarcoma, melanocyte, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have PTK2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 8422239, 23503467
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Melanocyte, Pubmed ID: 8247543, 9422762
Placenta, Pubmed ID: 15489334
T-cell, Pubmed ID: 7692878
Trachea, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-02-06
Question
Is this PB9674 anti-FAK/PTK2 antibody reactive to the isotypes of PTK2?
Verified Customer
Verified customer
Asked: 2018-03-01
Answer
The immunogen of PB9674 anti-FAK/PTK2 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human PTK2 (1024-1052aa AHALAVDAKNLLDVIDQARLKMLGQTRPH), identical to the related mouse and rat sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-03-01
Question
Is a blocking peptide available for product anti-FAK/PTK2 antibody (PB9674)?
H. Taylor
Verified customer
Asked: 2013-10-01
Answer
We do provide the blocking peptide for product anti-FAK/PTK2 antibody (PB9674). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-10-01
