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Product Info Summary
| SKU: | M00801-1 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-FAAH1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00801-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-FAAH1 Rabbit Monoclonal Antibody catalog # M00801-1. Tested in WB, IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FAAH1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00801-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
20F33
Isotype
IgG
Immunogen
A synthesized peptide derived from human FAAH1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00801-1 is reactive to FAAH in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
63 kDa
Calculated molecular weight
42778 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00801-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Positive Control
WB: human SH-SY5Y whole cell, mouse brain tissue, mouse pancreas tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of FAAH using anti-FAAH antibody (M00801-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: mouse brain tissue lysates,
Lane 3: mouse pancreas tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAAH antigen affinity purified monoclonal antibody (Catalog # M00801-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FAAH at approximately 63 kDa. The expected band size for FAAH is at 63 kDa.
Protein Target Info & Infographic
Gene/Protein Information For FAAH (Source: Uniprot.org, NCBI)
Gene Name
FAAH
Full Name
Fatty-acid amide hydrolase 1
Weight
42778 MW
Superfamily
amidase family
Alternative Names
Cyclin-dependent kinase 9;2.7.11.22;2.7.11.23;C-2K;Cell division cycle 2-like protein kinase 4;Cell division protein kinase 9;Serine/threonine-protein kinase PITALRE;Tat-associated kinase complex catalytic subunit;CDK9;CDC2L4, TAK; FAAH FAAH-1, PSAB fatty acid amide hydrolase fatty-acid amide hydrolase 1|anandamide amidohydrolase 1|fatty acid ester hydrolase|oleamide hydrolase 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FAAH, check out the FAAH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FAAH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FAAH1 Rabbit Monoclonal Antibody (M00801-1)
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