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Product Info Summary
| SKU: | A12667 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-EPN3/Epsin 3 Antibody
SKU/Catalog Number
A12667
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-EPN3/Epsin 3 Antibody (Catalog # A12667). Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EPN3/Epsin 3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12667)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human EPN3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A12667 is reactive to EPN3 in Human
Reconstitution
Calculated molecular weight
68222 MW
Background of Epsin 3
Belongs to the epsin family. 2 isoforms of the human protein are produced by alternative splicing.
Spradling K.D., J. Biol. Chem. 276:29257-29267(2001).
Ota T., Nat. Genet. 36:40-45(2004).
Mural R.J., Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12667 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts from K562 cells, using EPN3 antibody A12667.
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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using EPN3 antibody A12667.
Protein Target Info & Infographic
Gene/Protein Information For EPN3 (Source: Uniprot.org, NCBI)
Gene Name
EPN3
Full Name
Epsin-3
Weight
68222 MW
Superfamily
epsin family
Alternative Names
Epsin-3;EPS-15-interacting protein 3;EPN3; EPN3 epsin 3 epsin-3|EPS-15-interacting protein 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EPN3, check out the EPN3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EPN3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EPN3/Epsin 3 Antibody (A12667)
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No publications found for A12667
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-EPN3/Epsin 3 Antibody
Question
Would anti-EPN3/Epsin 3 antibody A12667 work for WB with fundus of stomach?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
According to the expression profile of fundus of stomach, EPN3 is highly expressed in fundus of stomach. So, it is likely that anti-EPN3/Epsin 3 antibody A12667 will work for WB with fundus of stomach.
Boster Scientific Support
Answered: 2020-04-22
Question
Would anti-EPN3/Epsin 3 antibody A12667 work on bovine IHC with placenta?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
Our lab technicians have not validated anti-EPN3/Epsin 3 antibody A12667 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-EPN3/Epsin 3 antibody A12667 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine placenta in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-12
Question
Is this A12667 anti-EPN3/Epsin 3 antibody reactive to the isotypes of EPN3?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
The immunogen of A12667 anti-EPN3/Epsin 3 antibody is Synthesized peptide derived from internal of human EPN3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-18
Question
We are currently using anti-EPN3/Epsin 3 antibody A12667 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-02-15
Answer
The anti-EPN3/Epsin 3 antibody (A12667) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-02-15
Question
Is there a BSA free version of anti-EPN3/Epsin 3 antibody A12667 available?
L. Carter
Verified customer
Asked: 2018-05-11
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-EPN3/Epsin 3 antibody A12667 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-05-11
