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Product Info Summary
| SKU: | A01311-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-L17A Receptor/IL17RA Antibody Picoband®
View all IL-17RA/IL-17R Antibodies
SKU/Catalog Number
A01311-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-L17A Receptor/IL17RA Antibody Picoband® catalog # A01311-2. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-L17A Receptor/IL17RA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01311-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human IL17RA recombinant protein (Position: K53-Q284). Human IL17RA shares 74.1% amino acid (aa) sequence identity with mouse IL17RA.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01311-2 is reactive to IL17RA in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
160 kDa
Calculated molecular weight
96122 MW
Background of IL-17RA/IL-17R
Interleukin 17 receptor A, also known as IL17RA and CDw217 (cluster of differentiation w217), is a human gene. The IL17R gene is mapped to 22q11.22-q11.23 by radiation hybrid analysis. Interleukin 17A (IL17A) is a proinflammatory cytokine secreted by activated T-lymphocytes. It is a potent inducer of the maturation of CD34-positive hematopoietic precursors into neutrophils. The protein encoded by this gene is a ubiquitous type I membrane glycoprotein that binds with low affinity to interleukin 17A. Interleukin 17A and its receptor play a pathogenic role in many inflammatory and autoimmune diseases such as rheumatoid arthritis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01311-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA, 0.1-0.5μg/ml, -,
Western blot, 0.1-0.5μg/ml, Human,
Positive Control
WB: human Hela whole cell, human HepG2 whole cell, human MCF-7 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IL17RA using anti-IL17RA antibody (A01311-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysate,
Lane 2: human HepG2 whole cell lysate,
Lane 3: human MCF-7 whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17RA antigen affinity purified polyclonal antibody (Catalog # A01311-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL17RA at approximately 160KD. The expected band size for IL17RA is at 96KD.
Protein Target Info & Infographic
Gene/Protein Information For IL17RA (Source: Uniprot.org, NCBI)
Gene Name
IL17RA
Full Name
Interleukin-17 receptor A
Weight
96122 MW
Alternative Names
Interleukin-17 receptor A;IL-17 receptor A;IL-17RA;CDw217;CD217;IL17RA;IL17R; Il17ra|AW538159, Cdw217, Il, Il17r, VDw21, VDw217|interleukin 17 receptor A|interleukin-17 receptor A|IL-17 receptor A|IL-17RA
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL17RA, check out the IL17RA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL17RA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-L17A Receptor/IL17RA Antibody Picoband® (A01311-2)
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Customer Q&As
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16 Customer Q&As for Anti-L17A Receptor/IL17RA Antibody Picoband®
Question
Is a blocking peptide available for product anti-L17A Receptor/IL17RA antibody (A01311-2)?
Verified Customer
Verified customer
Asked: 2020-03-09
Answer
We do provide the blocking peptide for product anti-L17A Receptor/IL17RA antibody (A01311-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-09
Question
I was wanting to use to test anti-L17A Receptor/IL17RA antibody A01311-2 on human uterus for research purposes, then I may be interested in using anti-L17A Receptor/IL17RA antibody A01311-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
N. Jackson
Verified customer
Asked: 2020-01-27
Answer
The products we sell, including anti-L17A Receptor/IL17RA antibody A01311-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-27
Question
We have been able to see staining in human t-cell. Do you have any suggestions? Is anti-L17A Receptor/IL17RA antibody supposed to stain t-cell positively?
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
From what I have seen in literature t-cell does express IL17RA. From what I have seen in Uniprot.org, IL17RA is expressed in blood, t-cell, uterus, placenta, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have IL17RA expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Placenta, Pubmed ID: 16785495
T-cell, Pubmed ID: 9367539
Uterus, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-12-12
Question
I was wanting to use your anti-L17A Receptor/IL17RA antibody for WB for human uterus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human uterus identification?
Verified Customer
Verified customer
Asked: 2019-12-10
Answer
It shows on the product datasheet, A01311-2 anti-L17A Receptor/IL17RA antibody has been validated for ELISA, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human uterus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-10
Question
I have attached the WB image, lot number and protocol we used for uterus using anti-L17A Receptor/IL17RA antibody A01311-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-19
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for uterus using anti-L17A Receptor/IL17RA antibody A01311-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-10
Question
I have a question about product A01311-2, anti-L17A Receptor/IL17RA antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01311-2 anti-L17A Receptor/IL17RA antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-23
Question
We purchased anti-L17A Receptor/IL17RA antibody for ELISA on placenta in a previous project. I am using human, and I plan to use the antibody for WB next. We need examining placenta as well as cervix carcinoma erythroleukemia in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
I looked at the website and datasheets of our anti-L17A Receptor/IL17RA antibody and it appears that A01311-2 has been tested on human in both ELISA and WB. Thus A01311-2 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-08-08
Question
We are currently using anti-L17A Receptor/IL17RA antibody A01311-2 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
C. Zhang
Verified customer
Asked: 2019-07-19
Answer
The anti-L17A Receptor/IL17RA antibody (A01311-2) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-19
Question
We are interested in using your anti-L17A Receptor/IL17RA antibody for positive regulation of interleukin-5 secretion studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-02-27
Answer
We appreciate your inquiry. This A01311-2 anti-L17A Receptor/IL17RA antibody is tested on human hela, hela whole cell lysate, hepg2 whole cell lysate. It is guaranteed to work for ELISA, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-02-27
Question
Is this A01311-2 anti-L17A Receptor/IL17RA antibody reactive to the isotypes of IL17RA?
Verified Customer
Verified customer
Asked: 2018-10-30
Answer
The immunogen of A01311-2 anti-L17A Receptor/IL17RA antibody is E. coli-derived human IL17RA recombinant protein (Position: K53-Q284). Human IL17RA shares 74.1% amino acid (aa) sequence identity with mouse IL17RA. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-10-30
Question
Do you have a BSA free version of anti-L17A Receptor/IL17RA antibody A01311-2 available?
Verified Customer
Verified customer
Asked: 2018-08-08
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-L17A Receptor/IL17RA antibody A01311-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-08-08
Question
My boss were content with the WB result of your anti-L17A Receptor/IL17RA antibody. However we have been able to see positive staining in t-cell isoform 2: secreted using this antibody. Is that expected? Could you tell me where is IL17RA supposed to be expressed?
S. Yang
Verified customer
Asked: 2018-03-23
Answer
Based on literature, t-cell does express IL17RA. Generally IL17RA expresses in isoform 1: cell membrane, isoform 2: secreted. Regarding which tissues have IL17RA expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Placenta, Pubmed ID: 16785495
T-cell, Pubmed ID: 9367539
Uterus, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-03-23
Question
Would anti-L17A Receptor/IL17RA antibody A01311-2 work for WB with uterus?
Verified Customer
Verified customer
Asked: 2018-02-01
Answer
According to the expression profile of uterus, IL17RA is highly expressed in uterus. So, it is likely that anti-L17A Receptor/IL17RA antibody A01311-2 will work for WB with uterus.
Boster Scientific Support
Answered: 2018-02-01
Question
Will A01311-2 anti-L17A Receptor/IL17RA antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Yang
Verified customer
Asked: 2016-10-20
Answer
As indicated on the product datasheet, A01311-2 anti-L17A Receptor/IL17RA antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-10-20
Question
I see that the anti-L17A Receptor/IL17RA antibody A01311-2 works with WB, what is the protocol used to produce the result images on the product page?
G. Mangal
Verified customer
Asked: 2014-03-31
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-03-31
