Anti-Phospho-EphB1/2 (Tyr594/604) Antibody

Product Name

Anti-Phospho-EphB1/2 (Tyr594/604) Antibody

View all EPHB1 Antibodies

SKU/Catalog Number

P02811

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-EphB1/2 (Tyr594/604) Antibody (Catalog # P02811). Tested in WB, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-EphB1/2 (Tyr594/604) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P02811)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Immunogen

Synthesized peptide derived from human EphB1/2 around the phosphorylation site of Y594/604.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P02811 is reactive to EPHB1 in Human, Mouse, Rat

Applications

P02811 is guaranteed for ELISA, IF, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

109885 MW

Background of EPHB1

Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells.

Ferrer, et al. (2003) Neuropathology & Applied Neurobiology 29: 23
Zhonghong Guan, et al. (1999) J Biol Chem, Vol. 274: 36200-36206
D.Margriet Ouwens1, et al. (2002)The EMBO Journal 21: 3782

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1/500 - 1/2000
Immunofluorescence: 1/200 - 1/1000
ELISA: 1/5000
Not yet tested in other applications.

Validation Images & Assay Conditions

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Western Blot analysis of HELA A431 cells using Phospho-EphB1/2 (Y594/604) Polyclonal antibody

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Western Blot analysis of Hela cells using Phospho-EphB1/2 (Y594/604) Polyclonal antibody

Gene/Protein Information For EPHB1 (Source: Uniprot.org, NCBI)

Alternative Names

Cek6; EC 2.7.10; EC 2.7.10.1; EK6; Elk; EPH receptor B1; eph tyrosine kinase 2; EphB1; EPH-like kinase 6; ephrin type-B receptor 1; EPHT2FLJ37986; Hek6; Net; NETHek6; soluble EPHB1 variant 1; Tyrosine-protein kinase receptor EPH-2 EPHB1 ELK, EPHT2, Hek6, NET EPH receptor B1 ephrin type-B receptor 1|EK6|EPH-like kinase 6|eph tyrosine kinase 2|neuronally-expressed EPH-related tyrosine kinase|soluble EPHB1 variant 1|tyrosine-protein kinase receptor EPH-2

For more info on EPHB1, check out the EPHB1 Infographic

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In this infographic, you will see the following information for EPHB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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