Click image to see more details
Product Info Summary
| SKU: | M00171-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ICAM1/Cd54 Rabbit Monoclonal Antibody
View all ICAM-1/CD54 Antibodies
SKU/Catalog Number
M00171-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ICAM1/Cd54 Rabbit Monoclonal Antibody catalog # M00171-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ICAM1/Cd54 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00171-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DOD-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ICAM1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00171-1 is reactive to ICAM1 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
29 kDa
Calculated molecular weight
57825 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00171-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Positive Control
WB: Raji cell
Validation Images & Assay Conditions
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Western blot analysis of ICAM1 expression in Raji cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For ICAM1 (Source: Uniprot.org, NCBI)
Gene Name
ICAM1
Full Name
Intercellular adhesion molecule 1
Weight
57825 MW
Superfamily
immunoglobulin superfamily
Alternative Names
Intercellular adhesion molecule 1;ICAM-1;Major group rhinovirus receptor;CD54;ICAM1; ICAM1 BB2, CD54, P3.58 intercellular adhesion molecule 1 intercellular adhesion molecule 1|ICAM-1|cell surface glycoprotein P3.58|epididymis secretory sperm binding protein|intercellular adhesion molecule 1 (CD54), human rhinovirus receptor|major group rhinovirus receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ICAM1, check out the ICAM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ICAM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ICAM1/Cd54 Rabbit Monoclonal Antibody (M00171-1)
Hello CJ!
M00171-1 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Anti-inflammatory effect of the blueberry anthocyanins malvidin-3-glucoside and malvidin-3-galactoside in endothelial cells
Cheng Y, Zhang Y, Song H, Feng J. Neural Regen Res. 2012 Jun 15;7(17):1312-7. Doi: 10.3969/J.Issn.1673-5374.2012.17.005. Intercellular Adhesion Molecule-1 Expression In The Hippocampal Ca1 Region Of Hyperlipidemic Rats With Chronic Cerebral Ischemia.
Yao Jh, Zhang Xs, Zheng Ss, Li Yh, Wang Lm, Wang Zz, Chu L, Hu Xw, Liu Kx, Tian Xf. World J Gastroenterol. 2009 Jul 14;15(26):3240-5. Prophylaxis With Carnosol Attenuates Liver Injury Induced By Intestinal Ischemia/Reperfusion.
Wang W, Zhao F, Fang Y, Li X, Shen L, Cao T, Zhu H. Crit Care. 2013 Mar 11;17(2):R44. Doi: 10.1186/Cc12558. Glycyrrhizin Protects Against Porcine Endotoxemia Through Modulation Of Systemic Inflammatory Response.
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-ICAM1/Cd54 Rabbit Monoclonal Antibody
Question
We need to test anti-ICAM1/Cd54 Rabbit Monoclonal antibody M00171-1 on human erythroleukemia for research purposes, then I may be interested in using anti-ICAM1/Cd54 Rabbit Monoclonal antibody M00171-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-13
Answer
The products we sell, including anti-ICAM1/Cd54 Rabbit Monoclonal antibody M00171-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-13
Question
We have observed staining in human cervix carcinoma. What should we do? Is anti-ICAM1/Cd54 Rabbit Monoclonal antibody supposed to stain cervix carcinoma positively?
T. Zhao
Verified customer
Asked: 2019-11-15
Answer
According to literature cervix carcinoma does express ICAM1. According to Uniprot.org, ICAM1 is expressed in lung, kidney, blood, plasma, cervix carcinoma, liver, leukemic t-cell, erythroleukemia, among other tissues. Regarding which tissues have ICAM1 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15572059
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Erythroleukemia, Pubmed ID: 23186163
Kidney, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 19159218, 24275569
Lung, Pubmed ID: 14702039
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2019-11-15
Question
Will M00171-1 anti-ICAM1/Cd54 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
You can see on the product datasheet, M00171-1 anti-ICAM1/Cd54 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-06
Question
My boss were satisfied with the WB result of your anti-ICAM1/Cd54 Rabbit Monoclonal antibody. However we have seen positive staining in plasma membrane using this antibody. Is that expected? Could you tell me where is ICAM1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-19
Answer
From literature, plasma does express ICAM1. Generally ICAM1 expresses in membrane. Regarding which tissues have ICAM1 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15572059
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Erythroleukemia, Pubmed ID: 23186163
Kidney, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 19159218, 24275569
Lung, Pubmed ID: 14702039
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2019-06-19
Question
Do you have a BSA free version of anti-ICAM1/Cd54 Rabbit Monoclonal antibody M00171-1 available?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ICAM1/Cd54 Rabbit Monoclonal antibody M00171-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-18
Question
Our lab want to know about using your anti-ICAM1/Cd54 Rabbit Monoclonal antibody for interleukin-4 and interleukin-13 signaling studies. Has this antibody been tested with western blotting on raji cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-04-01
Answer
We appreciate your inquiry. This M00171-1 anti-ICAM1/Cd54 Rabbit Monoclonal antibody is tested on raji cell lysate. It is guaranteed to work for IF, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-04-01
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for erythroleukemia using anti-ICAM1/Cd54 Rabbit Monoclonal antibody M00171-1. Let me know if you need anything else.
A. Anderson
Verified customer
Asked: 2018-01-30
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-30
Question
Is a blocking peptide available for product anti-ICAM1/Cd54 Rabbit Monoclonal antibody (M00171-1)?
Verified Customer
Verified customer
Asked: 2017-08-22
Answer
We do provide the blocking peptide for product anti-ICAM1/Cd54 Rabbit Monoclonal antibody (M00171-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-08-22
Question
Will anti-ICAM1/Cd54 Rabbit Monoclonal antibody M00171-1 work for WB with erythroleukemia?
Verified Customer
Verified customer
Asked: 2017-07-10
Answer
According to the expression profile of erythroleukemia, ICAM1 is highly expressed in erythroleukemia. So, it is likely that anti-ICAM1/Cd54 Rabbit Monoclonal antibody M00171-1 will work for WB with erythroleukemia.
Boster Scientific Support
Answered: 2017-07-10
Question
I was wanting to use your anti-ICAM1/Cd54 Rabbit Monoclonal antibody for WB for human erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human erythroleukemia identification?
M. Jones
Verified customer
Asked: 2016-09-14
Answer
As indicated on the product datasheet, M00171-1 anti-ICAM1/Cd54 Rabbit Monoclonal antibody has been tested for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-09-14
Question
We are currently using anti-ICAM1/Cd54 Rabbit Monoclonal antibody M00171-1 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?
P. Wu
Verified customer
Asked: 2016-09-14
Answer
The anti-ICAM1/Cd54 Rabbit Monoclonal antibody (M00171-1) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-09-14
Question
We ordered your anti-ICAM1/Cd54 Rabbit Monoclonal antibody for IF on leukemic t-cell in the past. I am using human, and I plan to use the antibody for WB next. I was wanting to use examining leukemic t-cell as well as liver in our next experiment. Could give a recommendation on which antibody would work the best for WB?
P. Johnson
Verified customer
Asked: 2016-09-06
Answer
I viewed the website and datasheets of our anti-ICAM1/Cd54 Rabbit Monoclonal antibody and it appears that M00171-1 has been tested on human in both IF and WB. Thus M00171-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2016-09-06
Question
I see that the anti-ICAM1/Cd54 Rabbit Monoclonal antibody M00171-1 works with WB, what is the protocol used to produce the result images on the product page?
B. Zhang
Verified customer
Asked: 2015-12-30
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-12-30
Question
Can you help my question with product M00171-1, anti-ICAM1/Cd54 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
G. Rodriguez
Verified customer
Asked: 2015-07-09
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00171-1 anti-ICAM1/Cd54 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-07-09
Question
I have attached the WB image, lot number and protocol we used for erythroleukemia using anti-ICAM1/Cd54 Rabbit Monoclonal antibody M00171-1. Please let me know if you require anything else.
C. Evans
Verified customer
Asked: 2015-04-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-04-08
Question
Is this M00171-1 anti-ICAM1/Cd54 Rabbit Monoclonal antibody reactive to the isotypes of ICAM1?
J. Li
Verified customer
Asked: 2013-11-28
Answer
The immunogen of M00171-1 anti-ICAM1/Cd54 Rabbit Monoclonal antibody is A synthesized peptide derived from human ICAM1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-11-28
