Anti-integrin beta 4 binding protein/EIF6 Antibody Picoband®

integrin beta 4 binding protein antibody

Boster Bio Anti-integrin beta 4 binding protein/EIF6 Antibody Picoband® catalog # PB9582. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: PB9582
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, ICC, WB

Product Name

Anti-integrin beta 4 binding protein/EIF6 Antibody Picoband®

View all integrin beta 4 binding protein Antibodies

SKU/Catalog Number

PB9582

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-integrin beta 4 binding protein/EIF6 Antibody Picoband® catalog # PB9582. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-integrin beta 4 binding protein/EIF6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9582)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human EIF6 recombinant protein (Position: N66-T210). Human EIF6 shares 99.3% amino acid (aa) sequence identity with both mouse and rat EIF6.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9582 is reactive to EIF6 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

26 kDa

Calculated molecular weight

26599 MW

Background of integrin beta 4 binding protein

EIF6 (Eukaryotic Translation Initiation Factor 6), also called EIF3A or ITGB4BP, is a human gene. By fluorescence in situ hybridization, Sanvito et al. (1998) mapped the ITGB4BP gene to 20q11.2. Ceci et al. (2003) demonstrated that the ribosomal 60S subunit is activated by release of EIF6. In the cytoplasm, EIF6 is bound to free 60S but not to 80S subunits. Furthermore, EIF6 interacts in the cytoplasm with RACK1, a receptor for activated protein kinase C. Gandin et al. (2008) demonstrated that mammalian eIF6 is required for efficient initiation of translation in vivo. Eif6-null mouse embryos were lethal at preimplantation. Heterozygous mice had 50% reduction of eIF6 levels in all tissues, and showed reduced mass of hepatic and adipose tissues due to a lower number of cells and to impaired G1/S cell cycle progression.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PB9582 is guaranteed for IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat
Immunocytochemistry, 0.5-1μg/ml
Western blot, 0.1-0.5μg/ml

Positive Control

WB: human Hela whole cell, human K562 whole cell, human 293T whole cell, human HepG2 whole cell, rat liver tissue, mouse liver tissue, mouse RAW2647 whole cell
IHC: Mouse Intestine tissue, Rat Testis tissue, Human Intestinal Cancer tissue
ICC: SMMC-7721 Cell, SW480 Cell

Validation Images & Assay Conditions

Gene/Protein Information For EIF6 (Source: Uniprot.org, NCBI)

Gene Name

EIF6

Full Name

Eukaryotic translation initiation factor 6

Weight

26599 MW

Superfamily

eIF-6 family

Alternative Names

Eukaryotic translation initiation factor 6 ;eIF-6 ;B (2)GCN homolog;B4 integrin interactor;CAB;p27 (BBP);EIF6 ;EIF3A, ITGB4BP ;OK/SW-cl.27; EIF6 CAB, EIF3A, ITGB4BP, b(2)gcn, eIF-6, p27(BBP), p27BBP eukaryotic translation initiation factor 6 eukaryotic translation initiation factor 6|B4 integrin interactor|eukaryotic translation initiation factor 3A|p27 beta-4 integrin-binding protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on EIF6, check out the EIF6 Infographic

EIF6 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for EIF6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-integrin beta 4 binding protein/EIF6 Antibody Picoband®

Question

Does anti-integrin beta 4 binding protein/EIF6 antibody PB9582 work for WB with liver?

Verified Customer

Verified customer

Asked: 2019-08-27

Answer

According to the expression profile of liver, EIF6 is highly expressed in liver. So, it is likely that anti-integrin beta 4 binding protein/EIF6 antibody PB9582 will work for WB with liver.

Boster Scientific Support

Answered: 2019-08-27

Question

We are currently using anti-integrin beta 4 binding protein/EIF6 antibody PB9582 for mouse tissue, and we are happy with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?

G. Collins

Verified customer

Asked: 2019-08-08

Answer

The anti-integrin beta 4 binding protein/EIF6 antibody (PB9582) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-08

Question

I see that the anti-integrin beta 4 binding protein/EIF6 antibody PB9582 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-09-20

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-09-20

Question

See below the WB image, lot number and protocol we used for liver using anti-integrin beta 4 binding protein/EIF6 antibody PB9582. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-04-19

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-04-19

Question

Does anti-integrin beta 4 binding protein/EIF6 antibody PB9582 work on horse ICC with liver?

O. Williams

Verified customer

Asked: 2016-11-28

Answer

Our lab technicians have not validated anti-integrin beta 4 binding protein/EIF6 antibody PB9582 on horse. You can run a BLAST between horse and the immunogen sequence of anti-integrin beta 4 binding protein/EIF6 antibody PB9582 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse liver in ICC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2016-11-28

Question

Do you have a BSA free version of anti-integrin beta 4 binding protein/EIF6 antibody PB9582 available?

S. Collins

Verified customer

Asked: 2015-11-12

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-integrin beta 4 binding protein/EIF6 antibody PB9582 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2015-11-12

Question

I was wanting to use your anti-integrin beta 4 binding protein/EIF6 antibody for WB for mouse liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse liver identification?

B. Moore

Verified customer

Asked: 2013-08-23

Answer

You can see on the product datasheet, PB9582 anti-integrin beta 4 binding protein/EIF6 antibody has been tested for IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse liver in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-08-23

Order DetailsPrice
PB9582

100μg

$370
PB9582-10ug

10μg sample (liquid)

$99
PB9582-Biotin

100 μg Biotin conjugated

$570
PB9582-Cy3

100 μg Cy3 conjugated

$570
PB9582-Dylight488

100 μg Dylight488 conjugated

$570
PB9582-Dylight550

100 μg Dylight550 conjugated

$570
PB9582-Dylight594

100 μg Dylight594 conjugated

$570
PB9582-FITC

100 μg FITC conjugated

$570
PB9582-HRP

100 μg HRP conjugated

$570
PB9582-APC

100 μg APC conjugated

$670
PB9582-PE

100 μg PE conjugated

$670
PB9582-iFluor647

100 μg iFluor647 conjugated

$670
PB9582-carrier-free

Carrier Free

$370

More conjugates/formats

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PB9582
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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