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Product Info Summary
| SKU: | M01727 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-eIF5A Rabbit Monoclonal Antibody
SKU/Catalog Number
M01727
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-eIF5A Rabbit Monoclonal Antibody catalog # M01727. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-eIF5A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01727)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FAO-5
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human eIF5A
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01727 is reactive to EIF5A in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
68 kDa
Calculated molecular weight
16832 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01727 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:5000-1:10000
IHC 1:50-1:200
ICC/IF 1:100-1:500
IP 1:50
FC 1:50
Positive Control
IHC: human uterus cancer tissue
ICC/IF: Hela cell
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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Immunofluorescent analysis of Hela cells, using eIF5A Antibody.
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Western blot analysis of eIF5A expression in Jurkat cell lysate.
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Immunohistochemical analysis of paraffin-embedded human uterus cancer, using eIF5A Antibody.
Protein Target Info & Infographic
Gene/Protein Information For EIF5A (Source: Uniprot.org, NCBI)
Gene Name
EIF5A
Full Name
Eukaryotic translation initiation factor 5A-1
Weight
16832 MW
Superfamily
eIF-5A family
Alternative Names
Eukaryotic translation initiation factor 5A-1;eIF-5A-1;eIF-5A1;Eukaryotic initiation factor 5A isoform 1;eIF-5A;Rev-binding factor;eIF-4D;EIF5A; EIF5A EIF-5A1, eIF-4D, eIF5AI, EIF5A eukaryotic translation initiation factor 5A eukaryotic translation initiation factor 5A-1|eukaryotic initiation factor 5A|rev-binding factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EIF5A, check out the EIF5A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EIF5A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-eIF5A Rabbit Monoclonal Antibody (M01727)
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No publications found for M01727
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-eIF5A Rabbit Monoclonal Antibody
Question
See below the WB image, lot number and protocol we used for right testis using anti-eIF5A Rabbit Monoclonal antibody M01727. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-14
Question
Will anti-eIF5A Rabbit Monoclonal antibody M01727 work for IF with right testis?
Verified Customer
Verified customer
Asked: 2017-08-08
Answer
According to the expression profile of right testis, EIF5A is highly expressed in right testis. So, it is likely that anti-eIF5A Rabbit Monoclonal antibody M01727 will work for IF with right testis.
Boster Scientific Support
Answered: 2017-08-08
Question
I see that the anti-eIF5A Rabbit Monoclonal antibody M01727 works with IF, what is the protocol used to produce the result images on the product page?
C. Kulkarni
Verified customer
Asked: 2013-09-18
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-09-18
