Product Info Summary
SKU: | A09801 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-CERKL/Ceramide Kinase Like Antibody
View all Ceramide Kinase Like Antibodies
SKU/Catalog Number
A09801
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-CERKL/Ceramide Kinase Like Antibody (Catalog # A09801). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CERKL/Ceramide Kinase Like Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09801)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human CERKL.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A09801 is reactive to CERKL in Human
Reconstitution
Calculated molecular weight
62622 MW
Background of Ceramide Kinase Like
Has no detectable ceramide-kinase activity. Overexpression of CERKL protects cells from apoptosis in oxidative stress conditions.
Tuson M., Am. J. Hum. Genet. 74:128-138(2004).
Bornancin F., Biochim. Biophys. Acta 1687:31-43(2005).
Ota T., Nat. Genet. 36:40-45(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09801 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from A549 cells, using CERKL antibody A09801.
Protein Target Info & Infographic
Gene/Protein Information For CERKL (Source: Uniprot.org, NCBI)
Gene Name
CERKL
Full Name
Ceramide kinase-like protein
Weight
62622 MW
Alternative Names
Ceramide kinase-like protein;CERKL; CERKL RP26 ceramide kinase like ceramide kinase-like protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CERKL, check out the CERKL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CERKL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CERKL/Ceramide Kinase Like Antibody (A09801)
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No publications found for A09801
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Customer Q&As
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5 Customer Q&As for Anti-CERKL/Ceramide Kinase Like Antibody
Question
Will anti-CERKL/Ceramide Kinase Like antibody A09801 work for WB with brain?
Verified Customer
Verified customer
Asked: 2020-03-20
Answer
According to the expression profile of brain, CERKL is highly expressed in brain. So, it is likely that anti-CERKL/Ceramide Kinase Like antibody A09801 will work for WB with brain.
Boster Scientific Support
Answered: 2020-03-20
Question
Would A09801 anti-CERKL/Ceramide Kinase Like antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
It shows on the product datasheet, A09801 anti-CERKL/Ceramide Kinase Like antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-21
Question
Is this A09801 anti-CERKL/Ceramide Kinase Like antibody reactive to the isotypes of CERKL?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
The immunogen of A09801 anti-CERKL/Ceramide Kinase Like antibody is Synthesized peptide derived from internal of human CERKL. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-10
Question
Is a blocking peptide available for product anti-CERKL/Ceramide Kinase Like antibody (A09801)?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
We do provide the blocking peptide for product anti-CERKL/Ceramide Kinase Like antibody (A09801). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-04
Question
We are currently using anti-CERKL/Ceramide Kinase Like antibody A09801 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
C. Carter
Verified customer
Asked: 2016-11-21
Answer
The anti-CERKL/Ceramide Kinase Like antibody (A09801) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-11-21