Anti-CERKL/Ceramide Kinase Like Antibody

Ceramide Kinase Like antibody

Boster Bio Anti-CERKL/Ceramide Kinase Like Antibody (Catalog # A09801). Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A09801
Size: 100 µl
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-CERKL/Ceramide Kinase Like Antibody

View all Ceramide Kinase Like Antibodies

SKU/Catalog Number

A09801

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-CERKL/Ceramide Kinase Like Antibody (Catalog # A09801). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CERKL/Ceramide Kinase Like Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09801)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human CERKL.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A09801 is reactive to CERKL in Human

Reconstitution

Calculated molecular weight

62622 MW

Background of Ceramide Kinase Like

Has no detectable ceramide-kinase activity. Overexpression of CERKL protects cells from apoptosis in oxidative stress conditions.

Tuson M., Am. J. Hum. Genet. 74:128-138(2004).
Bornancin F., Biochim. Biophys. Acta 1687:31-43(2005).
Ota T., Nat. Genet. 36:40-45(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A09801 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For CERKL (Source: Uniprot.org, NCBI)

Gene Name

CERKL

Full Name

Ceramide kinase-like protein

Weight

62622 MW

Alternative Names

Ceramide kinase-like protein;CERKL; CERKL RP26 ceramide kinase like ceramide kinase-like protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CERKL, check out the CERKL Infographic

CERKL infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CERKL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-CERKL/Ceramide Kinase Like Antibody

Question

Will anti-CERKL/Ceramide Kinase Like antibody A09801 work for WB with brain?

Verified Customer

Verified customer

Asked: 2020-03-20

Answer

According to the expression profile of brain, CERKL is highly expressed in brain. So, it is likely that anti-CERKL/Ceramide Kinase Like antibody A09801 will work for WB with brain.

Boster Scientific Support

Answered: 2020-03-20

Question

Would A09801 anti-CERKL/Ceramide Kinase Like antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-02-21

Answer

It shows on the product datasheet, A09801 anti-CERKL/Ceramide Kinase Like antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-02-21

Question

Is this A09801 anti-CERKL/Ceramide Kinase Like antibody reactive to the isotypes of CERKL?

Verified Customer

Verified customer

Asked: 2020-01-10

Answer

The immunogen of A09801 anti-CERKL/Ceramide Kinase Like antibody is Synthesized peptide derived from internal of human CERKL. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-01-10

Question

Is a blocking peptide available for product anti-CERKL/Ceramide Kinase Like antibody (A09801)?

Verified Customer

Verified customer

Asked: 2019-10-04

Answer

We do provide the blocking peptide for product anti-CERKL/Ceramide Kinase Like antibody (A09801). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-10-04

Question

We are currently using anti-CERKL/Ceramide Kinase Like antibody A09801 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?

C. Carter

Verified customer

Asked: 2016-11-21

Answer

The anti-CERKL/Ceramide Kinase Like antibody (A09801) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-11-21

Order DetailsPrice
A09801-100uL

100uL

$360

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A09801
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$360.00

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