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Product Info Summary
| SKU: | PA1894 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-p107/RBL1 Antibody Picoband®
SKU/Catalog Number
PA1894
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-p107/RBL1 Antibody catalog # PA1894. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p107/RBL1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1894)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human p107, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1894 is reactive to RBL1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
60 kDa
Calculated molecular weight
120847 MW
Background of p107
RBL1 (Retinoblastoma-like 1), also known as Cullular Proteins p107, CP107 and 107, is a protein that in humans is encoded by the RBL1 gene. Ewen et al. (1991)used a partial cDNA for human p107 to map the gene to 20q11. by fluorescence in situ hybridization. Huppi et al. (1996)found that the mouse Rbl1 gene maps to the distal end of chromosome 2, as does also the E2f1 gene. The protein encoded by this gene is similar in sequence and possibly function to the product of the retinoblastoma 1 (RB1) gene. The RB1 gene product is a tumor suppressor protein that appears to be involved in cell cycle regulation, as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. Both the RB1 protein and the product of this gene can form a complex with adenovirus E1A protein and SV40 Large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. In addition, both proteins can inhibit the transcription of cell cycle genes containing E2F binding sites in their promoters. Due to the sequence and biochemical similarities with the RB1 protein, it is though that the protein encoded by this gene may also be a tumor suppressor. Two transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1894 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: 239T Cell, JURKAT Cell
IHC: Human Intestinal Cancer Tissue, Rat Intestine Tissue
Validation Images & Assay Conditions
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Anti-p107 antibody, PA1894, Western blotting
Lane 1: 239T Cell Lysate
Lane 2: JURKAT Cell Lysate
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Anti-p107 antibody, PA1894, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
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Anti-p107 antibody, PA1894, IHC(P)
IHC(P): Rat Intestine Tissue
Protein Target Info & Infographic
Gene/Protein Information For RBL1 (Source: Uniprot.org, NCBI)
Gene Name
RBL1
Full Name
Retinoblastoma-like protein 1
Weight
120847 MW
Superfamily
retinoblastoma protein (RB) family
Alternative Names
Retinoblastoma-like protein 1;107 kDa retinoblastoma-associated protein;p107;pRb1;RBL1; RBL1 CP107, PRB1, p107 RB transcriptional corepressor like 1 retinoblastoma-like protein 1|107 kDa retinoblastoma-associated protein|retinoblastoma-like 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RBL1, check out the RBL1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RBL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-p107/RBL1 Antibody Picoband® (PA1894)
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Customer Q&As
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4 Customer Q&As for Anti-p107/RBL1 Antibody Picoband®
Question
We are currently using anti-p107/RBL1 antibody PA1894 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-05
Answer
The anti-p107/RBL1 antibody (PA1894) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-05
Question
Do you have a BSA free version of anti-p107/RBL1 antibody PA1894 available?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-p107/RBL1 antibody PA1894 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-08
Question
I have a question about product PA1894, anti-p107/RBL1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-23
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1894 anti-p107/RBL1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-23
Question
Does anti-p107/RBL1 antibody PA1894 work on monkey IHC with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-04-19
Answer
Our lab technicians have not tested anti-p107/RBL1 antibody PA1894 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-p107/RBL1 antibody PA1894 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey cervix carcinoma erythroleukemia in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-19
