Product Info Summary
SKU: | M03922-3 |
---|---|
Size: | 100 μl/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-eIF4A1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M03922-3
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-eIF4A1 Rabbit Monoclonal Antibody catalog # M03922-3. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-eIF4A1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03922-3)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
18E95
Isotype
IgG
Immunogen
A synthesized peptide derived from human eIF4A1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03922-3 is reactive to EIF4A1 in Human, Mouse, Rat
Applications
M03922-3 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
46 kDa
Calculated molecular weight
55990 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of EIF4A1 using anti-EIF4A1 antibody (M03922-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: rat PC-12 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF4A1 antigen affinity purified monoclonal antibody (Catalog # M03922-3) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF4A1 at approximately 46 kDa. The expected band size for EIF4A1 is at 46 kDa.
Protein Target Info & Infographic
Gene/Protein Information For EIF4A1 (Source: Uniprot.org, NCBI)
Gene Name
EIF4A1
Full Name
Eukaryotic initiation factor 4A-I
Weight
55990 MW
Superfamily
DEAD box helicase family
Alternative Names
ATP-dependent RNA helicase eIF4A-1; DDX2Aeukaryotic initiation factor 4A-I; EC 3.6.1; EC 3.6.4.13; EIF4A; EIF-4A; eIF-4A-I; eIF4A-I; eukaryotic initiation factor 4AI; eukaryotic translation initiation factor 4A; eukaryotic translation initiation factor 4A, isoform 1; eukaryotic translation initiation factor 4A1 EIF4A1 DDX2A, EIF-4A, EIF4A, eIF-4A-I, eIF4A-I eukaryotic translation initiation factor 4A1 eukaryotic initiation factor 4A-I|ATP-dependent RNA helicase eIF4A-1|eukaryotic initiation factor 4AI|eukaryotic translation initiation factor 4A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EIF4A1, check out the EIF4A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EIF4A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-eIF4A1 Rabbit Monoclonal Antibody (M03922-3)
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