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Product Info Summary
| SKU: | PA1636 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, IHC-F, WB |
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Product info
Product Name
Anti-Lamin B2/LMNB2 Antibody Picoband®
SKU/Catalog Number
PA1636
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Lamin B2/LMNB2 Antibody catalog # PA1636. Tested in IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Lamin B2/LMNB2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1636)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Lamin B2, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1636 is reactive to LMNB2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
70 kDa
Calculated molecular weight
67689 MW
Background of Lamin B2
Lamin B2, also called LMNB2, is a protein associated with laminopathies. The LMNB2 gene is mapped to the G-negative subtelomeric band p13.3 of chromosome 19 by in situ hybridization. The LMNB2 gene contains 12 exons. Common model organisms have been used in the study of Lamin B2 function. A conditional knockout mouse line, called Lmnb2, is generated as part of the International Knockout Mouse Consortium program-a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists. A highly sensitive procedure about mapping the start site of DNA replication in a 13.7-kb region of human chromosome 19 coding for lamin B2 is developed for the identification of the origin of bidirectional DNA synthesis in single-copy replicons of mammalian cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1636 is guaranteed for IHC, IHC-F, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat
Positive Control
WB: human Hela whole cell, human Hela (nucleus), human K562 (nucleus), human PC-3 whole cell, rat C6 whole cell, mouse NIH/3T3 whole cell
IHC: Rat Intestine tissue
IHC-F: rat intestine tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Lamin B2 using anti-Lamin B2 antibody (PA1636).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Hela (nucleus) lysates,
Lane 3: human K562 (nucleus) lysates,
Lane 4: human PC-3 whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lamin B2 antigen affinity purified polyclonal antibody (Catalog # PA1636) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lamin B2 at approximately 70 kDa. The expected band size for Lamin B2 is at 68 kDa.
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Figure 2. IHC analysis of Lamin B2 using anti-Lamin B2 antibody (PA1636).
Lamin B2 was detected in a paraffin-embedded section of Rat Intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Lamin B2 Antibody (PA1636) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Lamin B2 using anti-Lamin B2 antibody (PA1636).
Lamin B2 was detected in frozen section of rat intestine tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Lamin B2 Antibody (PA1636) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For LMNB2 (Source: Uniprot.org, NCBI)
Gene Name
LMNB2
Full Name
Lamin-B2
Weight
67689 MW
Superfamily
intermediate filament family
Alternative Names
Lamin-B2;LMNB2;LMN2; Lmnb2|lamin B2|lamin-B2|lamin B3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LMNB2, check out the LMNB2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LMNB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Lamin B2/LMNB2 Antibody Picoband® (PA1636)
Hello CJ!
PA1636 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Protection of Nrf2 against arsenite-induced oxidative damage is regulated by the cyclic guanosine monophosphate-protein kinase G signaling pathway
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Customer Q&As
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6 Customer Q&As for Anti-Lamin B2/LMNB2 Antibody Picoband®
Question
My question regarding product PA1636, anti-Lamin B2/LMNB2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1636 anti-Lamin B2/LMNB2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-18
Question
Will anti-Lamin B2/LMNB2 antibody PA1636 work on pig IHC-P with cervix carcinoma?
C. Collins
Verified customer
Asked: 2019-12-19
Answer
Our lab technicians have not validated anti-Lamin B2/LMNB2 antibody PA1636 on pig. You can run a BLAST between pig and the immunogen sequence of anti-Lamin B2/LMNB2 antibody PA1636 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig cervix carcinoma in IHC-P, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-19
Question
We are currently using anti-Lamin B2/LMNB2 antibody PA1636 for human tissue, and we are satisfied with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
The anti-Lamin B2/LMNB2 antibody (PA1636) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-09
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-Lamin B2/LMNB2 antibody PA1636. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-11-27
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-27
Question
I see that the anti-Lamin B2/LMNB2 antibody PA1636 works with IHC-P, what is the protocol used to produce the result images on the product page?
K. Singh
Verified customer
Asked: 2016-01-13
Answer
You can find protocols for IHC-P on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-01-13
Question
Is a blocking peptide available for product anti-Lamin B2/LMNB2 antibody (PA1636)?
E. Walker
Verified customer
Asked: 2013-06-11
Answer
We do provide the blocking peptide for product anti-Lamin B2/LMNB2 antibody (PA1636). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-06-11
