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Key=sku, value=A03922-2
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Key=created_at, value=2019-03-28 11:49:55
Key=updated_at, value=2024-10-27 05:02:06
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Key=status, value=Enabled
Key=name, value=Anti-EIF4A1/2/3 Antibody Picoband®
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Key=template, value=antibodies
Key=host, value=Rabbit
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Key=badge, value=free-antibody-validation
Key=price, value=370.0000
Key=weight, value=1.0000
Key=meta_title, value=Anti-EIF4A1/2/3 Antibody Picoband® | Bosterbio
Key=meta_description, value=Boster Bio Anti-EIF4A1/2/3 Antibody Picoband® catalog # A03922-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100μg/vial in Lyophilized form antibody.
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Key=url_key, value=anti-eif4a1-picoband-trade-antibody-a03922-2-boster
Key=url_path, value=anti-eif4a1-picoband-trade-antibody-a03922-2
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Key=image_label, value=Figure 1. IHC analysis of EIF4A using anti-EIF4A antibody (A03922-2).
EIF4A was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) f
Key=small_image_label, value=Figure 1. IHC analysis of EIF4A using anti-EIF4A antibody (A03922-2).
EIF4A was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) f
Key=thumbnail_label, value=Figure 1. IHC analysis of EIF4A using anti-EIF4A antibody (A03922-2).
EIF4A was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) f
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Key=size, value=100 μg/vial
Key=gene_full_name, value=eukaryotic translation initiation factor 4A1
Key=uniprot_id, value=P60842
Key=reconstitution, value=Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Key=applications, value=ELISA, Flow Cytometry, IF, IHC, ICC, WB
Key=reactivity, value=Human, Mouse, Rat
Key=form, value=Lyophilized
Key=concentration, value=Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Key=cross_reactivity, value=No cross-reactivity with other proteins.
Key=recommended_detection_systems, value=Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
Key=source_company, value=Boster A03922-2
Key=isotype, value=Rabbit IgG
Key=sample_size_available, value=30ug for $99, contact us for details
Key=related_product_skus, value=B0001-5,AR1156,AR0146
Key=product_category, value=Primary Antibodies
Key=research_category, value=Atherosclerosis, Cancer, Cancer Metabolism, Cardiovascular, Heart Disease, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism of Lipids and Lipoproteins, Pathways and Processes, Signal Transduction
Key=clonality, value=Polyclonal
Key=swatch_image, value=/a/0/a03913-3-cd6-primary-antibodies-wb-testing-1.jpg
Key=rating_value, value=100
Key=review_count, value=3
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Key=cj_related_products, value=mTOR Pathway:EKC2074^Regulation Of Translation:A00135T203|A00968T46^Tdp-43 Proteinopathies:A01001-1|A01001-2|PROTQ13148
Key=description, value=Boster Bio Anti-EIF4A1/2/3 Antibody Picoband® catalog # A03922-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=short_description, value=Boster Bio Anti-EIF4A1/2/3 Antibody Picoband® catalog # A03922-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=meta_keyword, value=Anti-EIF4A1/2/3 Antibody Picoband®
Key=contents, value=Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Key=background, value=Eukaryotic initiation factor 4A-I is a protein that in humans is encoded by the EIF4A1 gene. It is mapped to 17p13.1. EIF4A1 has been shown to interact with EIF4E and eukaryotic translation initiation factor 4 gamma.
Key=gene_name, value=EIF4A1
Key=synonyms, value=Eukaryotic initiation factor 4A-I; eIF-4A-I; eIF4A-I; ATP-dependent RNA helicase eIF4A-1; EIF4A1; DDX2A, EIF4A
Key=application_details, value=Western blot, 0.1-0.25μg/ml, Human,Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Key=subcellular_localization, value=Cytosol. Extracellular exosome. Cytoplasm. Eukaryotic translation initiation factor 4F complex. Membrane.
Key=tissue_specificity, value= Adipocytes.
Key=protein_function, value=ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
Key=application_notes, value=Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
Key=immunogen, value=E.coli-derived human EIF4A1/2/3 recombinant protein (Position: D32-I406 ).
Key=purification, value=Immunogen affinity purified.
Key=storage, value=Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Key=custom_attribute_1, value=46 kDa
Key=custom_attribute_2, value=WB: human Hela whole cell, human Jurkat whole cell, human A431 whole cell, human HEK293 whole cell, human HepG2 whole cell, human A549 whole cell, human U87 whole cell, human K562 whole cell, rat PC-12 whole cell, mouse spleen tissue, mouse thymus tissue, mouse lung tissue, mouse RAW2647 whole cell
IHC: human colon cancer tissue, mouse intestine tissue, human lung cancer tissue, human lung cancer tissue, rat intestine tissue
ICC/IF: U20S cell, U20S cell
FCM: HepG2 cell, U87 cell
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