Click image to see more details
Product Info Summary
| SKU: | PA2029 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-EIF2S1 Antibody Picoband®
View all eIF2 alpha/EIF2S1 Antibodies
SKU/Catalog Number
PA2029
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-EIF2S1 Antibody catalog # PA2029. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EIF2S1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2029)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human EIF2S1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2029 is reactive to EIF2S1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
94 kDa
Calculated molecular weight
36112 MW
Background of eIF2 alpha/EIF2S1
EIF2S1 (Eukaryotic Translation Initiation Factor 2, Subunit 1), also called EIF2-alpha, is a protein that in humans is encoded by the EIF2S1 gene. Hartz (2010) mapped the EIF2S1 gene to chromosome 14q23.3 based on an alignment of the EIF2S1 sequence with the genomic sequence (GRCh37). Ernst et al. (1987) stated that protein synthesis is inhibited due to phosphorylation of Eif2-alpha in hemin-deprived rabbit reticulocyte lysates. HeLa cells subjected to heat shock, serum deprivation, or interferon treatment followed by virus infection also show a correlation between EIF2-alpha phosphorylation and translational repression. Jacob et al. (1989) found that the alpha-Pal transcription factor bound to 2 palindromic sites within the EIF2-alpha promoter and was essential for transcription of the EIF2-alpha gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2029 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: COLO320 Cell, CEM Cell, RAJI Cell, HT1080 Cell,
IHC: Human Intestinal Cancer Tissue, Human Placenta Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-EIF2S1 antibody, PA2029, Western blotting
Lane 1: COLO320 Cell Lysate
Lane 2: CEM Cell Lysate
Lane 3: RAJI Cell Lysate
Lane 4: HT1080 Cell Lysate
Click image to see more details
Anti-EIF2S1 antibody, PA2029, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
Click image to see more details
Anti-EIF2S1 antibody, PA2029, IHC(P)
IHC(P): Human Placenta Tissue
Protein Target Info & Infographic
Gene/Protein Information For EIF2S1 (Source: Uniprot.org, NCBI)
Gene Name
EIF2S1
Full Name
Eukaryotic translation initiation factor 2 subunit 1
Weight
36112 MW
Superfamily
eIF-2-alpha family
Alternative Names
Eukaryotic translation initiation factor 2 subunit 1;Eukaryotic translation initiation factor 2 subunit alpha;eIF-2-alpha;eIF-2A;eIF-2alpha;EIF2S1;EIF2A; EIF2S1 EIF-2, EIF-2A, EIF-2alpha, EIF2, EIF2A eukaryotic translation initiation factor 2 subunit alpha eukaryotic translation initiation factor 2 subunit 1|eIF-2-alpha|eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EIF2S1, check out the EIF2S1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EIF2S1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EIF2S1 Antibody Picoband® (PA2029)
Hello CJ!
No publications found for PA2029
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-EIF2S1 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-EIF2S1 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Anti-EIF2S1 Antibody Picoband®
Question
Would anti-EIF2S1 antibody PA2029 work for WB with cervix carcinoma erythroleukemia?
J. Mitchell
Verified customer
Asked: 2020-05-01
Answer
According to the expression profile of cervix carcinoma erythroleukemia, EIF2S1 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-EIF2S1 antibody PA2029 will work for WB with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2020-05-01
Question
See attached the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-EIF2S1 antibody PA2029. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-04
Question
Is this PA2029 anti-EIF2S1 antibody reactive to the isotypes of EIF2S1?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
The immunogen of PA2029 anti-EIF2S1 antibody is A synthetic peptide corresponding to a sequence in the middle region of human EIF2S1(123-137aa KDEQLESLFQRTAWV). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-08
Question
We are currently using anti-EIF2S1 antibody PA2029 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
The anti-EIF2S1 antibody (PA2029) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-12
Question
Is a blocking peptide available for product anti-EIF2S1 antibody (PA2029)?
M. Carter
Verified customer
Asked: 2019-05-17
Answer
We do provide the blocking peptide for product anti-EIF2S1 antibody (PA2029). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-17
Question
Does PA2029 anti-EIF2S1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-11-15
Answer
As indicated on the product datasheet, PA2029 anti-EIF2S1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-11-15
Question
I was wanting to use your anti-EIF2S1 antibody for WB for human cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2018-02-05
Answer
As indicated on the product datasheet, PA2029 anti-EIF2S1 antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-05
