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Product Info Summary
| SKU: | A05757-3 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-EHHADH Antibody (C-term)
SKU/Catalog Number
A05757-3
Size
80 µl
Description
Boster Bio Anti-EHHADH Antibody (C-term) (Catalog # A05757-3). Tested in WB, IHC-P, Flow Cytometry application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-EHHADH Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05757-3)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This EHHADH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 662-690 amino acids from the C-terminal region of human EHHADH.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05757-3 is reactive to EHHADH in Human, Mouse, Rat
Applications
A05757-3 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
79495 Da
Background of EHHADH
EHHADH is a bifunctional enzyme and is one of the four enzymes of the peroxisomal beta-oxidation pathway. The N-terminal region of the encoded protein contains enoyl-CoA hydratase activity while the C-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:10-1:50
FC: 1:10-1:50
Validation Images & Assay Conditions
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Western blot analysis of EHHADH Antibody (C-term) in mouse liver (lane 1), kidney (lane 2) tissue lysates (35ug/lane). EHHADH (arrow) was detected using the purified Pab.
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Western blot analysis of lysates from mousr kidney, rat kidney and liver tissue (from left to right), using EHHADH Antibody (C-term). A05757-3 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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Formalin-fixed and paraffin-embedded human hepatocarcinoma with EHHADH Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Flow cytometric analysis of HepG2 cells using EHHADH Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protein Target Info & Infographic
Gene/Protein Information For EHHADH (Source: Uniprot.org, NCBI)
Gene Name
EHHADH
Full Name
Peroxisomal bifunctional enzyme
Weight
79495 Da
Alternative Names
enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase; enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase; LBFP; LBP; L-PBE; PBFEMGC120586; peroxisomal bifunctional enzyme3,2-trans-enoyl-CoA isomerase; peroxisomal enoyl-CoA hydratase; peroxisomal EHHADH ECHD, FRTS3, L-PBE, LBFP, LBP, MFE1, PBFE enoyl-CoA hydratase and 3-hydroxyacyl CoA dehydrogenase peroxisomal bifunctional enzyme|3,2-trans-enoyl-CoA isomerase|L-3-hydroxyacyl-CoA dehydrogenase|L-bifunctional protein, peroxisomal|PBE|enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase|enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase|multifunctional enzyme 1|peroxisomal enoyl-CoA hydratase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EHHADH, check out the EHHADH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EHHADH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EHHADH Antibody (C-term) (A05757-3)
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