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Product Info Summary
| SKU: | A05757-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PBFE EHHADH Antibody
SKU/Catalog Number
A05757-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-PBFE EHHADH Antibody catalog # A05757-1. Tested in WB applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PBFE EHHADH Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05757-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human EHHADH. AA range:476-525
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05757-1 is reactive to EHHADH in Human, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
79495 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05757-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of A549 cells using PBFE Polyclonal antibody.
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Western Blot (WB) analysis of specific cells using PBFE Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For EHHADH (Source: Uniprot.org, NCBI)
Gene Name
EHHADH
Full Name
Peroxisomal bifunctional enzyme
Weight
79495 MW
Alternative Names
Peroxisomal bifunctional enzyme;PBE;PBFE;Enoyl-CoA hydratase/3,2-trans-enoyl-CoA isomerase;4.2.1.17;5.3.3.8;3-hydroxyacyl-CoA dehydrogenase;1.1.1.35;EHHADH;ECHD; EHHADH ECHD, FRTS3, L-PBE, LBFP, LBP, MFE1, PBFE enoyl-CoA hydratase and 3-hydroxyacyl CoA dehydrogenase peroxisomal bifunctional enzyme|3,2-trans-enoyl-CoA isomerase|L-3-hydroxyacyl-CoA dehydrogenase|L-bifunctional protein, peroxisomal|PBE|enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase|enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase|multifunctional enzyme 1|peroxisomal enoyl-CoA hydratase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EHHADH, check out the EHHADH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EHHADH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PBFE EHHADH Antibody (A05757-1)
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No publications found for A05757-1
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Customer Q&As
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6 Customer Q&As for Anti-PBFE EHHADH Antibody
Question
I am looking for to test anti-PBFE antibody A05757-1 on human placenta synovium for research purposes, then I may be interested in using anti-PBFE antibody A05757-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-08
Answer
The products we sell, including anti-PBFE antibody A05757-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-08
Question
Will A05757-1 anti-PBFE antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
You can see on the product datasheet, A05757-1 anti-PBFE antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-13
Question
Would anti-PBFE antibody A05757-1 work for WB with placenta synovium?
Verified Customer
Verified customer
Asked: 2019-07-10
Answer
According to the expression profile of placenta synovium, EHHADH is highly expressed in placenta synovium. So, it is likely that anti-PBFE antibody A05757-1 will work for WB with placenta synovium.
Boster Scientific Support
Answered: 2019-07-10
Question
Will anti-PBFE antibody A05757-1 work on monkey WB with kidney?
Verified Customer
Verified customer
Asked: 2019-04-10
Answer
Our lab technicians have not tested anti-PBFE antibody A05757-1 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-PBFE antibody A05757-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey kidney in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-10
Question
We are currently using anti-PBFE antibody A05757-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on pig tissues as well?
E. Krishna
Verified customer
Asked: 2018-07-12
Answer
The anti-PBFE antibody (A05757-1) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-12
Question
Is there a BSA free version of anti-PBFE antibody A05757-1 available?
M. Huang
Verified customer
Asked: 2017-12-06
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-PBFE antibody A05757-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-12-06
