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Product Info Summary
| SKU: | A01583-3 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA |
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Product info
Product Name
Anti-Nag-1 GDF15 Antibody Peroxidase Conjugated
SKU/Catalog Number
A01583-3
Size
100ug
Form
Lyophilized
Description
Boster Bio Anti-Nag-1 GDF15 Antibody Peroxidase Conjugated (Catalog # A01583-3). Tested in ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.?Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Nag-1 GDF15 Antibody Peroxidase Conjugated (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01583-3)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-NAG-1 (C-terminal specific) antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminal end of human NAG-1 protein. A residue of cysteine was added to facilitate coupling to KLH.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01583-3 is reactive to GDF15 in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Observed Molecular Weight
42 kDa
Calculated molecular weight
46169 MW
Background of GDF-15
Non-steroidal anti-inflammatory drug (NSAID) activated gene (NAG-1) is a member of the transforming growth factor-beta (TGF-beta) superfamily. NAG-1 is also known as Macrophage Inhibitory Cytokine-1 (MIC-1), Growth Differentiation Factor 15 (GDF15), Placental Bone Morphogenetic Protein (PLAB), or Prostate Derived Factor (PDF). NAG-1 is expressed in human placenta, prostate and colon. It possesses antitumorigenic and proapoptotic activities. NAG-1 expression is dramatically increased in inflammation, injury and malignancy. Increase of NAG-1 expression is a feature of many cancers including breast, colon, pancreas and prostate. In a number of studies, NAG-1 expression was increased by a number of NSAIDs. This increase in expression may correlate with the chemopreventive effect NSAIDs seem to have with certain cancers. NAG-1 expression is also induced by PPAR gamma ligands and by several dietary compounds such as conjugated linoleic acids (CLAs), naturally occurring fatty acids in ruminant food products, indoles, epicatechin gallate, and genistein. Induced expression of NAG-1 results in stimulation of apoptosis and inhibition of cell growth. Inhibition of NAG-1 induced expression by small interference RNA (siRNA) results in repression of induced apoptosis. NAG-1 expression is regulated by a numbers of transcription factors such as ERG-1 and Sp1. EGR-1 may be necessary for NSAID-induced NAG-1 expression. The study of expression of NAG-1 proteins, including variants, is important to define their potential role as serum biomarkers for cancer diagnosis, treatment monitoring, epidemiology study, and nutrition surveys.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01583-3 is guaranteed for ELISA Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:10,000-1:50,000
IHC: 1:500-1:2,500
WB: 1:1,000-1:5,000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of GDF15 using anti-GDF15 antibody (A01583-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GDF15 antigen affinity purified polyclonal antibody (Catalog # A01583-3) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for GDF15.
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Figure 2. IHC analysis of GDF15 using anti-GDF15 antibody (A01583-3).
GDF15 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GDF15 Antibody (A01583-3) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of GDF15 using anti-GDF15 antibody (A01583-3).
GDF15 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GDF15 Antibody (A01583-3) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For GDF15 (Source: Uniprot.org, NCBI)
Gene Name
GDF15
Full Name
Growth/differentiation factor 15
Weight
46169 MW
Superfamily
TGF-beta family
Alternative Names
NAG1 peroxidase conjugation, HRP conjugated NAG1, NAG 1, NAG-1, GDF15, MIC1, MIC-1, GDF-15, PLAB, PTGFB, nonsteroidal anti-inflammatory drug-activated gene, NSAID-activated gene 1 protein, growth differentiation factor 15, macrophage inhibitory compound 1, Placental bone morphogenetic protein, Prostate differentiation factor GDF15 GDF-15, MIC-1, MIC1, NAG-1, PDF, PLAB, PTGFB growth differentiation factor 15 growth/differentiation factor 15|NRG-1|NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1|NSAID-activated gene 1 protein|NSAID-regulated gene 1 protein|PTGF-beta|macrophage inhibitory cytokine 1|non-steroidal anti-inflammatory drug-activated gene-1|placental TGF-beta|placental bone morphogenetic protein|prostate differentiation factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GDF15, check out the GDF15 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GDF15: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Nag-1 GDF15 Antibody Peroxidase Conjugated (A01583-3)
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Customer Q&As
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16 Customer Q&As for Anti-Nag-1 GDF15 Antibody Peroxidase Conjugated
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-Nag-1 antibody Peroxidase Conjugated A01583-3. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-05-01
Question
See below the WB image, lot number and protocol we used for placenta using anti-Nag-1 antibody Peroxidase Conjugated A01583-3. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-30
Question
Is there a BSA free version of anti-Nag-1 antibody Peroxidase Conjugated A01583-3 available?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Nag-1 antibody Peroxidase Conjugated A01583-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-03
Question
Is this A01583-3 anti-Nag-1 antibody Peroxidase Conjugated reactive to the isotypes of GDF15?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
The immunogen of A01583-3 anti-Nag-1 antibody Peroxidase Conjugated is anti-NAG-1 (C-terminal specific) antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminal end of human NAG-1 protein. A residue of cysteine was added to facilitate coupling to KLH. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-28
Question
I was wanting to use your anti-Nag-1 antibody Peroxidase Conjugated for WB for mouse placenta on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse placenta identification?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
It shows on the product datasheet, A01583-3 anti-Nag-1 antibody Peroxidase Conjugated has been validated for IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-05
Question
Does A01583-3 anti-Nag-1 antibody Peroxidase Conjugated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
You can see on the product datasheet, A01583-3 anti-Nag-1 antibody Peroxidase Conjugated as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-25
Question
My boss were well pleased with the WB result of your anti-Nag-1 antibody Peroxidase Conjugated. However we have been able to see positive staining in chorionic villus secreted using this antibody. Is that expected? Could you tell me where is GDF15 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
From literature, chorionic villus does express GDF15. Generally GDF15 expresses in secreted. Regarding which tissues have GDF15 expression, here are a few articles citing expression in various tissues:
Fibrosarcoma, Pubmed ID: 9348093
Muscle, Pubmed ID: 15489334
Placenta, Pubmed ID: 9375789, 9593718, 14702039
Boster Scientific Support
Answered: 2019-07-19
Question
Will anti-Nag-1 antibody Peroxidase Conjugated A01583-3 work on dog WB with chorionic villus?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
Our lab technicians have not validated anti-Nag-1 antibody Peroxidase Conjugated A01583-3 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Nag-1 antibody Peroxidase Conjugated A01583-3 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog chorionic villus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-21
Question
We have been able to see staining in human placenta. Are there any suggestions? Is anti-Nag-1 antibody Peroxidase Conjugated supposed to stain placenta positively?
Verified Customer
Verified customer
Asked: 2019-04-10
Answer
From what I have seen in literature placenta does express GDF15. From what I have seen in Uniprot.org, GDF15 is expressed in chorionic villus, placenta, fibrosarcoma, muscle, among other tissues. Regarding which tissues have GDF15 expression, here are a few articles citing expression in various tissues:
Fibrosarcoma, Pubmed ID: 9348093
Muscle, Pubmed ID: 15489334
Placenta, Pubmed ID: 9375789, 9593718, 14702039
Boster Scientific Support
Answered: 2019-04-10
Question
My question regarding product A01583-3, anti-Nag-1 antibody Peroxidase Conjugated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-12-13
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01583-3 anti-Nag-1 antibody Peroxidase Conjugated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-12-13
Question
We are currently using anti-Nag-1 antibody Peroxidase Conjugated A01583-3 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2018-09-06
Answer
The anti-Nag-1 antibody Peroxidase Conjugated (A01583-3) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-09-06
Question
We want to test anti-Nag-1 antibody Peroxidase Conjugated A01583-3 on mouse placenta for research purposes, then I may be interested in using anti-Nag-1 antibody Peroxidase Conjugated A01583-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Krishna
Verified customer
Asked: 2017-12-28
Answer
The products we sell, including anti-Nag-1 antibody Peroxidase Conjugated A01583-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-12-28
Question
Is a blocking peptide available for product anti-Nag-1 antibody Peroxidase Conjugated (A01583-3)?
A. Zhang
Verified customer
Asked: 2017-01-18
Answer
We do provide the blocking peptide for product anti-Nag-1 antibody Peroxidase Conjugated (A01583-3). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-01-18
Question
I see that the anti-Nag-1 antibody Peroxidase Conjugated A01583-3 works with WB, what is the protocol used to produce the result images on the product page?
C. Williams
Verified customer
Asked: 2015-09-11
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-09-11
Question
We have tried in the past anti-Nag-1 antibody Peroxidase Conjugated for WB on fibrosarcoma in a previous experiment. I am using mouse, and We want to use the antibody for IHC next. you antibody examining fibrosarcoma as well as chorionic villus in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?
P. Lewis
Verified customer
Asked: 2014-11-11
Answer
I looked at the website and datasheets of our anti-Nag-1 antibody Peroxidase Conjugated and I see that A01583-3 has been tested on mouse in both WB and IHC. Thus A01583-3 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-11-11
Question
Will anti-Nag-1 antibody Peroxidase Conjugated A01583-3 work for WB with placenta?
S. Wu
Verified customer
Asked: 2013-02-06
Answer
According to the expression profile of placenta, GDF15 is highly expressed in placenta. So, it is likely that anti-Nag-1 antibody Peroxidase Conjugated A01583-3 will work for WB with placenta.
Boster Scientific Support
Answered: 2013-02-06
