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Product Info Summary
| SKU: | A00023Y1173 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IHC, ICC, WB |
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Product info
Product Name
Anti-EGFR (Phospho-Y1173) Antibody
SKU/Catalog Number
A00023Y1173
Size
100ul
Form
Liquid
Description
Boster Bio Anti-EGFR (Phospho-Y1173) Antibody catalog # A00023Y1173. Tested in WB,ICC,IHC,IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EGFR (Phospho-Y1173) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00023Y1173)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Tyr1173 of human EGFR.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00023Y1173 is reactive to EGFR in Human
Reconstitution
Calculated molecular weight
134277 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00023Y1173 is guaranteed for IP, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000
ICC: 1:50-1:200
IHC: 1:50-1:200
Validation Images & Assay Conditions
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Figure 1. Immunocytochemistry staining of EGFR using Anti-EGFR (Phospho-Y1173) Antibody (A00023Y1173).
ICC staining (pY1173) in untreated A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde
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Figure 2. Immunocytochemistry staining of EGFR using Anti-EGFR (Phospho-Y1173) Antibody (A00023Y1173).
ICC staining Phospho-EGFR (pY1173) in untreated A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeProtein Target Info & Infographic
Gene/Protein Information For EGFR (Source: Uniprot.org, NCBI)
Gene Name
EGFR
Full Name
Epidermal growth factor receptor
Weight
134277 MW
Superfamily
protein kinase superfamily
Alternative Names
Epidermal growth factor receptor;2.7.10.1;Proto-oncogene c-ErbB-1;Receptor tyrosine-protein kinase erbB-1;EGFR;ERBB, ERBB1, HER1; EGFR ERBB, ERBB1, ERRP, HER1, NISBD2, PIG61, mENA epidermal growth factor receptor epidermal growth factor receptor|avian erythroblastic leukemia viral (v-erb-b) oncogene homolog|cell growth inhibiting protein 40|cell proliferation-inducing protein 61|epidermal growth factor receptor tyrosine kinase domain|erb-b2 receptor tyrosine kinase 1|proto-oncogene c-ErbB-1|receptor tyrosine-protein kinase erbB-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EGFR, check out the EGFR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EGFR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EGFR (Phospho-Y1173) Antibody (A00023Y1173)
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