Click image to see more details
Product Info Summary
| SKU: | A08756 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-EGFL6 Antibody Picoband®
SKU/Catalog Number
A08756
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-EGFL6 Antibody Picoband® catalog # A08756. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EGFL6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08756)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human EGFL6 recombinant protein (Position: K354-D535).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A08756 is reactive to EGFL6 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
62 kDa
Calculated molecular weight
61.317kDa
Background of EGFL6
EGFL6 is a member of the epidermal growth factor (EGF) repeat superfamily. Members of this superfamily are characterized by the presence of EGF-like repeats and are often involved in the regulation of cell cycle, proliferation, and developmental processes. The gene product contains a signal peptide, suggesting that it is secreted; an EGF repeat region consisting of 4 complete EGF-like repeats and 1 partial EGF-like repeat, 3 of which have a calcium-binding consensus sequence; an arg-gly-asp integrin association motif; and a MAM domain, which is believed to have an adhesive function. This gene is expressed early during development, and its expression has been detected in lung and meningioma tumors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08756 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human U-87MG whole cell, human A431 whole cell, human HL-60 whole cell, human K562 whole cell, human THP-1 whole cell, human Hela whole cell, rat liver tissue, mouse thymus tissue, mouse lung tissue, mouse liver tissue, mouse SP20 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of EGFL6 using anti-EGFL6 antibody (A08756).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human HL-60 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: human THP-1 whole cell lysates,
Lane 6: human Hela whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGFL6 antigen affinity purified polyclonal antibody (Catalog # A08756) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EGFL6 at approximately 62KD. The expected band size for EGFL6 is at 62KD.
Click image to see more details
Figure 2. Western blot analysis of EGFL6 using anti-EGFL6 antibody (A08756).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse thymus tissue lysates,
Lane 3: mouse lung tissue lysates,
Lane 4: mouse liver tissue lysates,
Lane 5: mouse SP20 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGFL6 antigen affinity purified polyclonal antibody (Catalog # A08756) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EGFL6 at approximately 62KD. The expected band size for EGFL6 is at 62KD.
Protein Target Info & Infographic
Gene/Protein Information For EGFL6 (Source: Uniprot.org, NCBI)
Gene Name
EGFL6
Full Name
Epidermal growth factor-like protein 6
Weight
61.317kDa
Superfamily
nephronectin family
Alternative Names
Epidermal growth factor-like protein 6; EGF-like protein 6; MAM and EGF domains-containing gene protein; EGFL6; MAEG; PP648; UNQ281/PRO320 EGFL6 MAEG, W80 EGF like domain multiple 6 epidermal growth factor-like protein 6|EGF repeat-containing protein 6|EGF-like protein 6|MAM and EGF domain containing|MAM and EGF domains-containing gene protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EGFL6, check out the EGFL6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EGFL6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EGFL6 Antibody Picoband® (A08756)
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Customer Q&As
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4 Customer Q&As for Anti-EGFL6 Antibody Picoband®
Question
Will anti-EGFL6 antibody A08756 work on bovine ELISA with brain?
Verified Customer
Verified customer
Asked: 2019-03-07
Answer
Our lab technicians have not validated anti-EGFL6 antibody A08756 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-EGFL6 antibody A08756 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine brain in ELISA, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-03-07
Question
We are currently using anti-EGFL6 antibody A08756 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2018-01-09
Answer
The anti-EGFL6 antibody (A08756) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-09
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-EGFL6 antibody A08756. Let me know if you need anything else.
E. Li
Verified customer
Asked: 2016-08-04
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-08-04
Question
I see that the anti-EGFL6 antibody A08756 works with WB, what is the protocol used to produce the result images on the product page?
M. Krishna
Verified customer
Asked: 2016-01-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-01-25
